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通过自动化固相提取进行微尺度库的并行净化

Michael Wleklinski¹, Paige M Carpenter¹, Kevin D Dykstra¹

¹Department of Discovery Chemistry, Merck & Co., Inc., Rahway, NJ 07065, USA.

SLAS technology

|February 29, 2024

概括

高通量实验 (HTE) 加快了药物发现,但净化仍然是一个瓶. 一种新的自动化固相提取 (SPE) 方法显著提高了化合物的纯度,以加速药物开发.

科学领域:

药用化学医学化学
过程化学过程化学
药物发现药物发现药物发现

背景情况:

高通量实验 (HTE) 对于加速药物发现至关重要,使快速反应优化和大型化合物库的合成成为可能.
尽管HTE取得了进展,但合成化合物的净化仍然是一个重要的瓶,耗费宝贵的时间和资源,并阻碍了制造,测试,设计 (DMT) 循环.
现有的净化方法往往缺乏与现代药物发现工作流程的需求相匹配的效率和可扩展性.

研究的目的:

开发和验证通过HTE生成的复合库的通用并行净化策略.
解决药物发现工作流程中持续存在的净化瓶问题.
提高通过HTE生产的复合图书馆的效率,可持续性和纯度.

主要方法:

使用固相提取 (SPE) 实现并行净化工作流.
自动化SPE流程以实现与HTE库合成的无集成.
将开发的方法应用于微尺度HTE格式中通过胺基键合合成的多种化合物库.

主要成果:

通过自动化SPE工作流程,通过复合物纯度显著升级.
展示了并行净化方法的效率和稳定性.
成功地将净化工作流与HTE库合成集成,简化了整个药物发现过程.

结论:

描述的自动化SPE方法为HTE驱动的药物发现中的净化挑战提供了更有效和可持续的解决方案.
这种用户友好的工作流显著提高了化合物的纯度,加速了DMT循环,并促进了新疗法的开发.
该方法广泛适用于使用HTE技术合成的各种复合库.

关键词:

分析的分析的分析的自动化自动化自动化自动化自动化在这里,HTE是HTE.同步净化并行净化平行合成并行合成

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