Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Isolation01:24

DNA Isolation

39.0K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
39.0K
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

573
In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
573

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

An Ultra-High Throughput Hydrogen-Deuterium Exchange Workflow Using Acoustic Ejection Mass Spectrometry for Studying Peptide Solution Structural Conformation.

Analytical chemistry·2026
Same author

Developing Pharmaceutically Relevant Pd-Catalyzed C-N Coupling Reactivity Models Leveraging High-Throughput Experimentation.

Journal of the American Chemical Society·2025
Same author

Heterocyclic Assembly: An Underutilized Disconnection with Potential to Maximize High Fsp<sup>3</sup> Chemical Space Exploration.

ACS medicinal chemistry letters·2025
Same author

Rapid Affinity and Microsomal Stability Ranking of Crude Mixture Libraries of Histone Deacetylase Inhibitors.

ACS medicinal chemistry letters·2024
Same author

Radical Chlorination of Non-Resonant Heterobenzylic C-H Bonds and High-Throughput Diversification of Heterocycles.

Chem·2024
Same author

Development of High-Throughput Experimentation Approaches for Rapid Radiochemical Exploration.

Journal of the American Chemical Society·2024

相关实验视频

Updated: Jul 1, 2025

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

10.6K

通过自动化固相提取进行微尺度库的并行净化.

Michael Wleklinski1, Paige M Carpenter1, Kevin D Dykstra1

  • 1Department of Discovery Chemistry, Merck & Co., Inc., Rahway, NJ 07065, USA.

SLAS technology
|February 29, 2024
PubMed
概括
此摘要是机器生成的。

高通量实验 (HTE) 加快了药物发现,但净化仍然是一个瓶. 一种新的自动化固相提取 (SPE) 方法显著提高了化合物的纯度,以加速药物开发.

关键词:
分析的 分析的 分析的自动化自动化自动化自动化自动化在这里,HTE是HTE.同步净化并行净化平行合成并行合成

更多相关视频

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

351
An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
10:08

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

Published on: May 21, 2021

4.2K

相关实验视频

Last Updated: Jul 1, 2025

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

10.6K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

351
An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
10:08

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

Published on: May 21, 2021

4.2K

科学领域:

  • 药用化学 医学化学
  • 过程化学 过程化学
  • 药物发现 药物发现 药物发现

背景情况:

  • 高通量实验 (HTE) 对于加速药物发现至关重要,使快速反应优化和大型化合物库的合成成为可能.
  • 尽管HTE取得了进展,但合成化合物的净化仍然是一个重要的瓶,耗费宝贵的时间和资源,并阻碍了制造,测试,设计 (DMT) 循环.
  • 现有的净化方法往往缺乏与现代药物发现工作流程的需求相匹配的效率和可扩展性.

研究的目的:

  • 开发和验证通过HTE生成的复合库的通用并行净化策略.
  • 解决药物发现工作流程中持续存在的净化瓶问题.
  • 提高通过HTE生产的复合图书馆的效率,可持续性和纯度.

主要方法:

  • 使用固相提取 (SPE) 实现并行净化工作流.
  • 自动化SPE流程以实现与HTE库合成的无集成.
  • 将开发的方法应用于微尺度HTE格式中通过胺基键合合成的多种化合物库.

主要成果:

  • 通过自动化SPE工作流程,通过复合物纯度显著升级.
  • 展示了并行净化方法的效率和稳定性.
  • 成功地将净化工作流与HTE库合成集成,简化了整个药物发现过程.

结论:

  • 描述的自动化SPE方法为HTE驱动的药物发现中的净化挑战提供了更有效和可持续的解决方案.
  • 这种用户友好的工作流显著提高了化合物的纯度,加速了DMT循环,并促进了新疗法的开发.
  • 该方法广泛适用于使用HTE技术合成的各种复合库.