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使用6种人类细胞类型的广泛覆盖试验的向电池,对56种结构多样化的PFAS进行危害和风险表征

Lucie C Ford¹, Hsing-Chieh Lin¹, Han-Hsuan D Tsai¹

¹Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX 77843, USA.

|February 29, 2024

概括

这项研究使用人体细胞模型来评估和多醇基物质 (PFAS) 的健康风险. 一些PFAS表现出细胞特异性活性,约20%的暴露边际,表明潜在的健康危害.

科学领域:

环境科学环境科学
毒理学毒理学毒理学
细胞生物学细胞生物学

背景情况:

和多醇基物质 (PFAS) 是持久的,生物累积的环境污染物.
广泛使用导致人类和环境的流行,引发严重的健康问题.
大多数PFAS的数据有限,这阻碍了监管风险评估.

研究的目的:

用人体体外试验测试来研究PFAS的结构-生物活性关系.
通过比较生物活性与暴露估计来描述潜在的健康风险.
为决策者提供有关PFAS危险的信息.

主要方法:

测试了来自6种人类细胞类型的8个子类的56个PFAS (iPSC衍生的肝细胞,神经元,心肌细胞,初级肝细胞,内皮细胞,HepG2).
使用了度-反应测定 (0.1-100μM).
使用经验和预测的暴露数据来推导的暴露边际 (MOE).

主要成果:

某些PFAS表现出细胞特异性活动,强调需要多样化的体外模型.
没有明确的特定类别的分类出现,尽管观察到一些链条长度和结构趋势.
大约20%的测试PFAS的MOE<1,这表明进一步调查的优先级更高.

结论:

一个基于人类细胞的模型汇编可以估计PFAS生物活性,与人类暴露数据进行比较.
细胞特异性生物活性凸显了PFAS风险评估的复杂性.
对于PFAS来说,建立强大的结构-生物活性关系仍然具有挑战性.

关键词:

集团化集团化集团化集团化在体外 (in vitro) 的情况下.风险投资额度的边际.在PFAS中,我们可以使用PFAS.风险的表征风险的表征.

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