比较暴露于不同功能组的聚乙烯纳米塑料对男性生殖系统的影响和机制
Xiao Gao1, Ke Xu1, Wanting Du1
1Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China.
The Science of the total environment
|February 29, 2024
概括
环境老化改变了纳米塑料的毒性. 具有功能群的聚烯纳米粒子 (PS-NPs),特别是氨基基基修饰,通过诱导氧化应激并通过PI3K-AKT-mTOR通路破坏精子生产,损害了雄性小鼠的生殖系统.
科学领域:
- 环境科学 环境科学
- 毒理学 毒理学 毒理学
- 生殖生物学 生殖生物学
背景情况:
- 环境老化改变了纳米塑料的特性,包括表面电荷和功能组.
- 这些变化可以显著影响纳米塑料对生物系统的毒理影响.
- 了解纳米塑料的衰老对于评估它们的环境和健康风险至关重要.
研究的目的:
- 研究聚乙烯纳米粒子 (PS-NP) 衰老和功能化的对哺乳动物生殖系统的影响.
- 在雄性小鼠中评估未经修改的,碳素和氨基功能化的PS-NPs的特定毒理作用.
- 阐明纳米塑料诱导的生殖毒性背后的分子机制.
主要方法:
- 暴露C57BL/6雄性小鼠5毫克/公斤/天的PS-NP与不同的功能组45天.
- 评估生殖参数,包括丸指数,精管形态,精子细胞数和精子质量.
- 使用基因本体学 (GO) 和基因和基因组 (KEGG) 京都百科全书进行蛋白质组分析. 路径丰富分析.
- 确认关键信号通路的参与,特别是PI3K-AKT-mTOR通路.
主要成果:
- PS-NPs诱导了氧化应激,降低了丸指数,破坏了生殖管道,并减少了精子数量.
- 在暴露于PS-NPs的小鼠中观察到精子形的增加.
- GO和KEGG分析显示了蛋白质运输,RNA剪接和mTOR信号通路中的丰富.
- 证实PI3K-AKT-mTOR通路过度激活,可能导致通过过度分化减少精子干细胞.
- 氨基功能化PS-NPs表现出最高的毒性,其次是氧功能化PS-NPs,然后是未修改的PS-NPs.
结论:
- 纳米塑料的衰老和功能化显著影响生殖毒性.
- 带正电荷的氨基功能化PS-NP对男性生殖系统构成最大的风险.
- PI3K-AKT-mTOR途径在调解纳米塑料诱导的生殖毒性方面发挥着至关重要的作用.
- 这项研究强调了在风险评估中考虑纳米塑料衰老的重要性,并了解它们的毒性机制.
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