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太平洋白Litopenaeus vannamei中CpG寡氧核酸和Toll类受体之间的相互作用

Xuerui Gong¹, Feng Hu¹, Jingjie Hu²

¹MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao, 266003, China; Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution, Ocean University of China, Sanya, 57202, China.

Developmental and comparative immunology

|February 29, 2024

概括

CpG oligodeoxynucleotides (ODNs) 与中的托尔类受体 (TLR) 相互作用,激活免疫信号通路. 这项研究阐明了这些相互作用,对于理解免疫力和CpG ODN功能至关重要.

科学领域:

免疫学免疫学免疫学
海洋生物学海洋生物学
分子生物学分子生物学

背景情况:

CpG oligodeoxynucleotides (ODNs) 是一种具有免疫调节作用的新型辅助剂.
在Litopenaeus vannamei中的收费类受体 (TLR) 识别CpG ODN,启动免疫反应.

研究的目的:

调查各种CpG ODNs和不同的类Toll-like受体 (LvTLRs) 之间的相互作用.
了解这些相互作用在Litopenaeus vannamei中激活的下游信号通路.

主要方法:

构建11个LvTLRs-pCMV重组组合物 (rLvTLRs) 的过程.
在人类胚胎脏293T (HEK293T) 细胞中进行双 luciferase 记者测定.
生物层干涉测量 (BLI) 用于亲和分析.

主要成果:

每个LvTLR激活了多个下游基因 (NF-κB,CREB,ISRE,IL-6,TNF-α,Myc),诱导了关键的信号通路.
大多数CpG ODN与多个LvTLR绑定,每个LvTLR都识别了各种CpG ODN,特别是A和B类.
BLI证实了特定CpGODN (2216, 2006, 2143, 21425) 与TLR蛋白的剂量依赖性亲缘关系.

结论:

CpG ODN和LvTLR之间的相互作用对于的CpG ODN诱导的免疫调节至关重要.
这些发现为研究LvTLR激活和Litopenaeus vannamei.中CpG ODN的免疫机制提供了基础.

关键词:

在CpG中使用的Oligodeoxynucleotides.这是天生的免疫力.利托佩内亚斯 (Litopenaeus vannamei) 是一个水族群.收费类受体的收费类受体

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