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阿扎西提丁-赫斯佩雷丁组合诱导人类白血病细胞中S相细胞周期停止和细胞亡

Kuan-Ming Lai^1,2, Ruo-Han Tseng², Yu-Hung Shih²

¹Program in Translational Medicine, National Chung Hsing University, Taichung, Taiwan, R.O.C.

Anticancer research

|February 29, 2024

概括

5-阿扎西丁 (Aza) 和素 (Hes) 的组合通过诱导细胞亡和细胞循环停止来协同抑制白血病细胞的增殖. 这种Aza-Hes组合显示出作为抗白血病治疗的潜力.

科学领域:

生物化学生化学
分子生物学分子生物学
癌症研究癌症研究

背景情况:

化疗药物,如用于白血病的5-azacytidine (Aza),可能会引起不良反应.
天然化合物hesperetin (Hes) 是一种有前途的抗癌辅助疗法.

研究的目的:

为了研究5-azacytidine (Aza) 和hesperetin (Hes) 对急性白血病细胞系的联合作用.
阐明这种联合治疗在白血病进展中的作用.

主要方法:

用Aza和Hes单独或组合治疗HL-60和U937白血病细胞.
细胞增殖,细胞亡和细胞周期使用各种测试和流细胞计分析.
与细胞亡和细胞周期相关的蛋白质表达通过西式涂抹进行了评估.
使用Chou-Talalay方法量化了协同效应.

主要成果:

无论是Aza还是Hes单一治疗,都会以剂量依赖的方式降低白血病细胞活力.
阿扎-赫斯组合通过诱导亡和S相细胞循环停止来协同抑制U937细胞增殖.
该组合调节了基激活蛋白激酶 (MAPK) 途径蛋白质,降低了p-ERK和p-c-Jun N-终端激酶的调节,同时提高了p-p38.

结论:

阿扎-赫斯组合诱导细胞亡和S相细胞循环停止,可能通过MAPK通路.
这种组合代表了治疗白血病的有希望的治疗策略.

关键词:

在白血病中,白血病.通过MAPK路径.灭症 (apoptosis) 是一种死亡的过程.亚扎西提丁是一种阿扎西提丁.没有希望,没有希望.分子机制的分子机制.

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