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Auricularia auricula 聚糖对高级糖化最终产品 (AGEs) 的潜在抑制作用

Pin Gong¹, Shuya Pei¹, Hui Long¹

¹School of Food science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China; School of Biological and Pharmaceutical Sciences, Shaanxi University of Science and Technology, Xi'an 710021, China.

International journal of biological macromolecules

|February 29, 2024

概括

耳膜耳膜的多糖化合物AAP-2S抑制了先进的糖化终产品 (AGEs) 的形成和氧化损伤. 它还调节了减少细胞纤维化的关键途径,显示出作为天然抗AGE剂的潜力.

科学领域:

生物化学生物化学
自然产品化学自然产品化学
细胞生物学细胞生物学

背景情况:

先进的糖化终产物 (AGEs) 有助于细胞损伤和纤维化.
自然化合物正在被探索,因为它们有潜力减轻AGE形成和相关病理.
耳耳的多糖是众所周知的各种生物活性.

研究的目的:

为了研究一种新型的 Auricularia auricula 多糖的抗糖化作用,AAP-2S.
阐明AAP-2S在防止AGE形成和细胞损伤方面的潜在机制.
评估AAP-2S作为天然抗AGE剂和食品添加剂的潜力.

主要方法:

从Auricularia auricula中提取和描述AAP-2S.
在体外测定使用BSA-果糖模型来评估AGE抑制和蛋白质保护.
具有CML诱导的细胞模型 (HK-2细胞) 用于研究代谢变化和途径调节.
基于GC-MS/MS的代谢学,用于分析代谢概况.
西方涂抹或类似的技术来分析RAGE/TGF-β/NOX4通路.

主要成果:

在体外,AAP-2S显著抑制了AGE的形成.
AAP-2S减轻了蛋白质的氧化损伤,保留了硫基并防止了结构变化.
聚糖体表现出金属化性质.
代谢分析揭示了AAP-2S在细胞模型中调节的代谢概况.
通过调节RAGE/TGF-β/NOX4通路,AAP-2S减轻了糖化和改善了细胞纤维化.

结论:

AAP-2S是一种来自Auricularia auricula的新型多糖,具有显著的抗糖化和抗氧化特性.
该化合物有效地保护蛋白质免受糖化诱导的损伤和结构变化.
通过调节RAGE/TGF-β/NOX4通路,AAP-2S显示了缓解细胞纤维化的潜力.
这些发现支持探索auricularia auricula多糖作为天然抗AGEs剂和功能性食品添加剂.

关键词:

抗糖化的抗糖化.耳朵中的耳朵 (Auricularia auricula) 是一种多糖化合物.在BSA-果糖模型中.代谢学代谢学代谢学在RAGE/TGF-β/NOX4路径中.

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