不同交叉连接剂对混合奇托/原基的作用,用于潜在的组织工程应用
Pablo Sánchez-Cid1, María Alonso-González1, Mercedes Jiménez-Rosado1
1Departmento de Ingeniería Química, Facultad de Química, Escuela Politécnica Superior, Universidad de Sevilla, 41012 Sevilla, Spain.
这项研究探讨了用于组织工程 (TE) 的奇托和原水凝. 基尼和EDC/NHS交联显著改善了水凝的性能,显示了TE应用的潜力.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 聚合物化学 聚合物化学
背景情况:
- 组织工程需要具有机械强度的生物相容支架.
- 来自生物聚合物的水凝是发展支架的有希望的候选人.
- 素和原基 (CH/CG) 具有潜力,但需要通过交叉链接进行优化.
研究的目的:
- 评估CH/CG水凝的微观结构,风湿学,功能和生物特性.
- 为了比较天然 (d-果糖,基尼,转胺酶) 和合成 (EDC/NHS) 交联剂的疗效.
- 确定最佳的交叉连接策略,以提高组织工程中的CH/CG水凝性能.
主要方法:
- 福里埃变换红外光谱法 (FTIR) 来确认交联反应.
- 微观结构和风湿学性能的表征.
- 功能性和体外生物性质的评估.
主要成果:
- FTIR证实了所有药物的成功交叉连接反应.
- 与基尼 (G) 和EDC/NHS的交叉连接表明,与其他药物相比,水凝的性能得到了更好的改善.
- 在与G和EDC-NHS交联的CH / CG水凝中,在基水凝上观察到显著的增强.
结论:
- 吉尼和EDC/NHS是基托和原蛋白水凝的高效交叉连接剂.
- 优化的CH/CG水凝在组织工程应用中显示出有前途的潜力.
- 对G,T和EDC-NHS交联系统进行进一步的调查是对TE有必要的.
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