对温度敏感的R基因介导抗性的转录组学发现了一种WAKL10蛋白相互作用网络
Katherine Noel1,2, Ivan R Wolf3, David Hughes4
1Centre for Agriculture, Food and Environmental Management, University of Hertfordshire, Hatfield, AL10 9AB, UK. knoel@lspb.eu.
Scientific reports
|February 29, 2024
概括
植物对像Leptosphaeria maculans这样的真菌病原体的耐药性显示出温度敏感性. 这项研究揭示了Brassica napus R基因Rlm7和Rlm4如何对温度有不同的反应,影响了疾病耐药性和可持续农业.
科学领域:
- 植物病理学 植物病理学
- 分子遗传学 分子遗传学
- 农作物科学 农作物科学
背景情况:
- 植物免疫反应的温度敏感性影响作物产量.
- 了解不同温度下的R基因功能对于可持续农业至关重要.
- 像Leptosphaeria maculans这样的无形病原体对油菜 (Brassica napus) 构成重大威胁.
研究的目的:
- 为了研究Brassica napus对Leptosphaeria maculans的温度依赖的抵抗机制.
- 为了比较温度敏感 (Rlm7) 和耐温度 (Rlm4) 抵抗等位基因的分子反应.
- 识别参与温度调节植物防御的宿主基因和途径.
主要方法:
- 在不同温度下对Brassica napus入侵线Topas-Rlm7和Topas-Rlm4的基因表达差异分析.
- 蛋白质与蛋白质相互作用网络分析.
- 基因本体学的丰富分析.
主要成果:
- 对Leptosphaeria maculans的耐药性在Topas-Rlm7中对温度敏感,但在Topas-Rlm4中具有弹性.
- 1,646个宿主基因在两条线上都对温度的反应显示出差异性表达.
- 在25°C时确定了Topas-Rlm7特有的WAKL10蛋白相互作用网络.
- 在Rlm4网络中,WRKY22被确定为VPS60.1的潜在目标.
- 膀介导的运输与防御通道有关.
结论:
- Rlm7-1等位基赋予了对温度敏感的抗性,而Rlm4则提供了弹性.
- 不同的基因表达和网络相互作用突出了温度在R基因功能中的作用.
- 在Rlm7中因效应器触发免疫的失调会影响与囊泡相关的对L. maculans的防御.
相关概念视频
01:56Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
02:45Responses to Heat and Cold Stress
13.5K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.5K
02:24Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
01:23Types of RNA
63.7K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.7K