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相关概念视频

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K
Lineage Commitment01:21

Lineage Commitment

3.0K
Commitment is the  process whereby stem cells:
3.0K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.1K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.1K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

3.2K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.2K
Hematopoiesis01:21

Hematopoiesis

5.3K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
5.3K
Stem Cell Niche01:26

Stem Cell Niche

5.1K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.1K

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Updated: Jul 1, 2025

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
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Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

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在造血干细胞承诺期间对Smarca5表达的差异性要求.

Tereza Turkova1, Juraj Kokavec1, Tomas Zikmund1

  • 1Hematology Laboratories, BIOCEV; 1st Faculty of Medicine, Charles University, Vestec, Czech Republic.

Communications biology
|February 29, 2024
PubMed
概括
此摘要是机器生成的。

SWI/SNF ATPase SMARCA5 (SNF2H) 对于造血细胞的发育至关重要. 一个低形态基因基因拯救了发育缺陷,并揭示了SMARCA5的存在.

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Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
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Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
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Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
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Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
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科学领域:

  • 分子生物学分子生物学
  • 血液形成 血液形成 血液形成
  • 染色体重塑 染色体重塑 的方法

背景情况:

  • 造血细胞的形成取决于ISWI ATPase SMARCA5 (SNF2H) 染色体重塑复合体.
  • Smarca5无条件和有条件的小鼠模型显示了各种表型,从胚胎致死性到较不严重的器官特异性缺陷.

研究的目的:

  • 通过低形态等位基 (S5tg) 来研究SMARCA5在血液形成中的功能.
  • 确定SMARCA5基因剂量对胚胎发育和造血干细胞分化的影响.

主要方法:

  • 使用Smarca5低形态 (S5tg) 和淘汰赛小鼠模型.
  • 使用Cre-lox系统 (hCD2iCre,Vav1iCre) 进行组织特异性基因删除.
  • 分析了造血干细胞和原始细胞种群和分化潜力.

主要成果:

  • 在S5tg基因组中,救出了血液形成中的发育停滞和淘汰模型中的致命表型.
  • 通过Vav1iCre驱动的S5tg小鼠显示出血造干细胞和前代细胞的积累.
  • 在这些细胞中观察到受损的淋巴细胞系入口和分化,与正常的骨髓细胞发育形成鲜明对比.

结论:

  • SMARCA5基因剂量会影响造血干细胞功能和淋巴细胞分化.
  • 低SMARCA5表达允许正常的胚胎发育,但改变了淋巴细胞在造血干细胞中的进入.