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生命早期的疫苗接种重新编程了新生儿骨髓状细胞的新陈代谢和功能

Yingying Chen^1,2,3,4, Hui Li^1,2, Jie Zhou^1,2

¹Institute of Pediatric Health and Disease, Department of Neonatology, Guangzhou Key Laboratory of Neonatal Intestinal Diseases, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

|March 1, 2024

概括

在新生小鼠中,Bacillus Calmette-Guérin (BCG) 疫苗接种减少了免疫抑制性髓状细胞. 这种BCG诱导的免疫细胞成熟可能会提供对免疫系统疾病的长期保护.

科学领域:

免疫学免疫学免疫学
新生儿发育的新生儿发展
疫苗学疫苗学疫苗学

背景情况:

新生儿免疫对于预防感染和免疫系统疾病至关重要.
早期疫苗接种对新生儿免疫细胞的确切影响仍然不完全理解.
髓衍生抑制细胞 (MDSCs) 是不成熟的髓细胞,在新生儿中具有免疫抑制功能.

研究的目的:

研究Bacillus Calmette-Guérin (BCG) 疫苗接种对新生儿骨髓性衍生抑制细胞 (MDSC) 的影响.
阐明BCG影响新生儿骨髓状细胞功能的潜在分子机制.
探索早期生活中BCG诱导的免疫调节的潜在长期益处.

主要方法:

单细胞转录组分析,以描述新生儿髓状细胞中的基因表达变化.
代谢物分析,以确定BCG疫苗接种引起的代谢变化.
功能性测试以评估MDSCs在BCG暴露后的免疫抑制能力.
药理上抑制关键信号通路 (mTOR,糖解) 以验证发现.

主要成果:

在小鼠中,BCG疫苗接种显著降低了新生儿MDSCs的免疫抑制功能.
在新生儿髓状细胞中,BCG诱导了mTOR/HIF1a信号通路的上调.
增强的葡萄糖分解被确定为新生儿髓状细胞中BCG影响的关键代谢途径.
抑制糖解或mTOR信号反转BCG对新生儿髓状细胞的影响.

结论:

接种BCG疫苗有助于新生儿髓状细胞的功能成熟.
mTOR/HIF1a信号通路和增强的葡萄糖分解调解BCG对新生儿髓状细胞的影响.
早期的BCG疫苗接种可能会建立更强大的免疫系统,有助于在以后的生命中保护免疫系统.

关键词:

细菌卡尔梅特盖林葡萄糖溶解是什么 mTOR 信号传输系统骨髓蛋白衍生的抑制细胞.

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