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使用人类口腔微生物群构建一个多功能体外培养选平台

Kengo Sasaki¹, Yasunobu Takeshima¹, Ayami Fujino¹

¹Bio Palette Co., Ltd, Kobe, Japan.

Environmental microbiology reports

|March 1, 2024

概括

使用Bio Palette口腔介质 (BPOM) 的新型模拟模型有效模仿人类口腔微生物群. 这种模型显示出对抗生素和益生菌对口腔病原体如Porphyromonas和Fusobacterium的测试有前途.

科学领域:

微生物学微生物学
口腔健康口腔健康
生物膜研究生物膜研究

背景情况:

人口微生物群在口腔健康中起着至关重要的作用.
特定的致病细菌,Porphyromonas和Fusobacterium,与口腔疾病有关.
现有的模拟模型可能无法准确地代表复杂的口腔环境.

研究的目的:

开发和验证人类口腔微生物群的模拟模型.
在这个模型中评估抗生素和益生菌的疗效.
研究营养度对致病性细菌生长的影响.

主要方法:

开发一种低葡萄糖和的仿真介质 (Bio Palette口服介质 - BPOM).
在酸 (HA) 磁盘上培养口腔微生物群.
与其他介质 (GAM,改性GAM,粘素) 的BPOM比较.
测试西塔弗洛克萨和米诺环素化的抑制作用.
评估益生菌 (Streptococcus salivarius,Limosilactobacillus fermentum) 的影响. 这是一个很好的方法.

主要成果:

与其他介质相比,BPOM显著增加了Porphyromonas和Fusobacterium的比率.
该模型准确地反映了原始唾液样本中发现的细菌比率.
西塔弗洛克萨在低剂量时显示出比米诺环素更强的抑制P. gingivalis.
益生菌有效地降低了目标致病性细菌的比率.

结论:

BPOM作为模拟人类口腔微生物群的多功能和有效平台.
该模型适用于评估口腔疾病的治疗干预措施.
在BPOM中低碳和营养条件促进了关键口腔病原体的生长,有助于治疗评估.

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