相关实验视频

Updated: Jul 1, 2025

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
00:07

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

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多参数屏幕优化了免疫沉

Shaoshuai Xie1, Leila Saba1, Hua Jiang2

  • 1European Research Institute for the Biology of Ageing, University Medical Centre Groningen, Groningen, 9713AV, The Netherlands.

BioTechniques
|March 1, 2024
PubMed
概括

这项研究引入了一种高含量选方法,以快速优化免疫沉 (IP) 条件. 这种方法通过稳定样本准备过程中的相互作用来增强蛋白质复合物的捕获,从而改善互动组映射.

关键词:
亲和关系 蛋白质组学复杂论的复杂学互动组学 互动组学 互动组学大分子组件的组合.

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相关概念视频

Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
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