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牛和猪自然感染 Mycobacterium tuberculosis 综合体的淋巴结粒瘤中的巨细胞极化

Fernanda Larenas-Muñoz¹, Mohamed G Hamed², Inés Ruedas-Torres¹

¹Department of Anatomy and Comparative Pathology and Toxicology, Pathology and Immunology Group (UCO-PIG), UIC Zoonosis y Enfermedades Emergentes ENZOEM, University of Córdoba, International Excellence Agrifood Campus 'CeiA3', Córdoba, Spain.

Veterinary pathology

|March 1, 2024

概括

巨细胞两极分化不同于牛和猪牛结核病. 牛在早期阶段表现出M1巨细胞的优势,而猪在整个颗粒瘤发育过程中表现出M2极化.

科学领域:

兽医免疫学兽医免疫学
病理学病理学病理学
细胞生物学细胞生物学

背景情况:

动物结核病是由Mycobacterium结核综合体 (MTC) 引起的.
颗粒瘤是特征性病变,巨细胞在它们的发育中起着至关重要的作用.
巨细胞表现出可塑性,分化为促炎 (M1) 或抗炎 (M2) 现型.

研究的目的:

调查自然感染MTC的牛和猪的淋巴结粒瘤内的巨细胞两极分化.
为了比较M1和M2巨细胞标记物的表达在两种物种的不同颗粒瘤阶段.

主要方法:

结核颗粒瘤的微观分类,分为四个阶段.
骨髓细胞标记物的免疫组合化学分析 (CD172a/calprotectin).
对M1 (iNOS/CD68/CD107a) 和M2 (Arg1/CD163) 巨细胞极化标记物的分析.

主要成果:

牛在早期细粒瘤阶段表达M1标记物 (iNOS,CD68) 的更高.
在所有颗粒瘤阶段,猪表现出更高的M2标志物 (Arg1) 表达.
仅在猪粒瘤中观察到CD107a (M1),主要是在早期阶段.

结论:

在早期MTC细粒瘤发育期间,M1巨细胞两极分化在牛中占主导地位.
在MTC颗粒瘤形成的所有阶段,M2巨细胞极化在猪中占主导地位.
巨细胞两极分化的特定物种差异影响了牛结核病的发病.

关键词:

这里是牛群.这种情况通常发生在颗粒状质瘤 (granuloma) 上.巨细胞是一个巨细胞.猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪两极分化是一种极化.结核病是一种肺结核病.

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