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Updated: Jul 1, 2025

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A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
Published on: March 17, 2023
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在没有预期激发光的情况下进行NIR-II光成像
Aiyan Ji1,2, Hongyue Lou2, Jiafeng Li2
1Department of Pharmacy, School of Pharmacy, Fudan University Shanghai 201203 China zhaoweili@fudan.edu.cn.
Chemical science
|March 1, 2024
概括
研究人员使用一种新型染料开发了白色发光二极管 (LED) 激发的近红外II (NIR-II) 成像. 这种方法可以清晰可视化小瘤和血管,指导手术切除.
科学领域:
- 生物医学光学 生物医学光学
- 医疗成像医学成像
- 光成像技术 光成像技术
背景情况:
- 第二次近红外窗 (NIR-II) 成像通常使用激光二极管来激发.
- 由于缺乏合适的光学探头,阻碍了白光激发的NIR-II成像.
- 现有的方法,如氨酸绿 (ICG) 在激光照射下遭受显著的光灭.
研究的目的:
- 开发和评估多波长白发光二极管 (LED) 激发的NIR-II成像技术.
- 为了引入一种新的蓝移 NIR-II 染料,TPA-TQT,用于白光激发.
- 评估该系统对生物医学成像和图像引导手术的性能.
主要方法:
- 使用了一种新的蓝移NIR-II染料 (TPA-TQT) 与多波长白色LED.
- 将白色LED激发与单波长LED和传统激光激发进行比较.
- 评估了血管和淋巴毛细血管的成像分辨率.
- 评估了系统在监测后肢缺血-再输和淋巴炎炎症模型中的有效性.
- 在携带瘤的小鼠中进行了白色LED激发的NIR-II光成像引导手术 (FIGS).
主要成果:
- 与808nm激光照射相比,白色LED系统显示出最小的光灭.
- 多波长LED激发提供了比单波长LED低的背景噪声.
- 实现了高分辨率的图像,识别了血管 (103微米) 和淋巴毛细血管 (129.8微米).
- 该系统成功地监测了诸如缺血-再输和淋巴炎症等生理过程.
- 白色LED激发的NIR-II图清楚地划出了小转移性瘤病变 (∼350微米),并指导了手术切除.
结论:
- 基于多波长白色LED的NIR-II成像是一种可行的激光激发替代方案.
- 这种新的系统提供了更好的染料稳定性和成像性能.
- 这项技术在精确的瘤划分和图像引导手术方面具有显著的潜力.
- 白色LED激发的NIR-II成像对各种生物医学应用具有前景.
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