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前核转移拯救了体外培养的小鼠二次毛囊的不良胚胎发育

Antonia Christodoulaki^1,2, Haitang He^1,2,3, Min Zhou^1,2

¹Ghent-Fertility and Stem cell Team (G-FaST), Department for Reproductive Medicine, Ghent University Hospital, Ghent, Belgium.

Human reproduction open

|March 1, 2024

概括

前核转移 (PNT) 通过利用体内成熟的细胞质成功恢复了试管培养 (IVG) 鼠标卵细胞中的胚胎发育. 这种技术导致了活着的,肥沃的后代的诞生,证明了其改善卵子质量的潜力.

科学领域:

生殖生物学生殖生物学
发展生物学发展生物学
辅助生殖技术辅助生殖技术

背景情况:

试管卵泡培养是一种潜在的生育保存方法,但试管培养 (IVG) 卵子由于细胞质无能,表现出比体内培养的卵子质量较低.
鼠标模型对于优化IVG卵细胞培养和了解发育问题至关重要.

研究的目的:

为了调查前核转移 (PNT) 是否可以克服由细胞质因素引起的IVG小鼠卵细胞中的胚胎发育停滞.
评估来自PNT的后代的发育潜力和生育能力.

主要方法:

二次小鼠毛囊在体外培养了9天,以产生IVG卵细胞.
与体内对照对象相比,IVG卵细胞的成熟率,直径,释放量和线粒体潜力都得到了评估.
PNT涉及将从激活的IVG卵细胞中的前核转移到受精在体内生长的卵细胞的细胞质中.
产生的胚胎经过基因分析并转移给代孕母亲.

主要成果:

与对照人群相比,IVG卵细胞的直径显著减少,释放能力和线粒体膜潜力显著减少.
对IVG卵细胞的基激活导致了显著降低的裂变和胚胎细胞分裂率.
在PNT后,IVG衍生的胚胎中的胚芽细胞形成率恢复到86.9%,与对照组相比.
在80%的分析胚胎中,PNT产生了具有正常染色体配置的活生生的,肥沃的后代.

结论:

PNT有效地克服了IVG小鼠卵子细胞的细胞质缺陷,恢复了正常的胚胎发育.
该技术有望改善胚胎的可用性,特别是对于具有低成熟效率的人类IVG卵细胞.
需要对人类卵细胞进行进一步的验证,以确定PNT的临床相关性.

关键词:

IVG IVG IVG IVG IVG IVG IVG IVG IVG IVG IVG IVG IVG 胚胎发育过程中的胚胎发育.保护生育能力保护生育能力毛囊的发育毛囊的发育核转移转移是核转移的一个过程.卵细胞质量卵细胞质量

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