相关实验视频

Updated: Jun 19, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

ExpoSeq:简化分析来自抗体发现活动的高通量测序数据

Christoffer V Sørensen¹, Nils Hofmann¹, Puneet Rawat²

¹Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark.

Bioinformatics advances

|March 1, 2024

概括

ExpoSeq简化了用于抗体发现的高通量测序 (HTS) 数据分析. 这种用户友好的工具使得没有编程技能的研究人员能够有效地探索和可视化复杂的HTS数据.

科学领域:

分子生物学分子生物学
生物信息学是一种生物信息学.
免疫学免疫学免疫学

背景情况:

高通量测序 (HTS) 对于推进抗体显示技术至关重要.
从抗体发现活动中分析HTS数据带来了重大的计算挑战.
缺乏可访问,用户友好的软件阻碍了研究人员没有广泛的编程专业知识的广泛采用.

研究的目的:

介绍ExpoSeq,一个用于处理和可视化HTS数据的直观软件工具.
为了使具有有限编程知识的科学家能够有效地分析抗体发现数据.
为了弥合复杂的HTS数据和分子生物学中的实际应用之间的差距.

主要方法:

开发一个名为ExpoSeq.的用户友好的软件管道.
实施用于探索,处理和可视化HTS数据的工具.
通过GitHub和PyPI进行分发,以方便安装 (pip或克隆).

主要成果:

在抗体发现过程中,ExpoSeq为HTS数据分析提供了一个可访问的平台.
该工具允许用户通过初学者的知识来探索和可视化复杂的数据集.
为缺乏高级编程技能的研究人员提供专家级数据处理.

结论:

在抗体研究中,ExpoSeq使高通量测序数据的分析民主化.
该软件通过简化数据解释来提高显示技术的实用性.
授权更广泛的科学家利用HTS进行抗体发现和开发.

更多相关视频

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...