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数据集用于选择稳定的参考基因,用于精确的定量基因表达分析:对性别差异化和确定的影响
Hwa Jin Lee1,2, Ki Tae Kim3, Min Sun Kim2
1CellQua, Inc, Seongnam 13595, South Korea.
Data in brief
|March 1, 2024
概括
这项研究确定了稳定的基因参考基因,用于精确的基因表达分析,在银尖四 (Hasemania nana). 建议使用RPL5,EF1A,PICALM和TUBB,而GAPDH不适用于RT-qPCR.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 水生生物学 水生生物学
背景情况:
- 使用RT-qPCR精确的基因表达分析依赖于稳定的参考基因.
- 选择适当的参考基因对于实验有效性至关重要,特别是在不同的组织和性别之间.
- 银尖 (Hasemania nana) 是一种新兴的鱼类研究模型生物.
研究的目的:
- 为了评估9个候选参考基因的表达稳定性在银尖四角.
- 确定不同组织 (大脑,生殖腺,尾) 和性别的RT-qPCR分析中最可靠的参考基因.
- 为未来关于银尖四角动物的性别分化和决定研究提供基础数据.
主要方法:
- 九个候选参考基因被选择用于稳定性分析.
- 基因表达稳定性在雄性和雌性银尖四角动物的大脑,生殖腺和尾翼组织中进行了评估.
- 稳定性使用geNorm,NormFinder,BestKeeper和RankAggreg算法进行分析.
主要成果:
- RPL5,EF1A和PICALM被确定为女性在各种组织中稳定的参考基因.
- TUBB和ITGB2在不同组织的男性中显示稳定性.
- 在特定组织中,TUBB,ITGB2,RPL5和PICALM被认为适用于两性,而GAPDH则始终不稳定.
结论:
- 根据组织和性别,建议在银尖四中进行RT-qPCR,使用特定的参考基因 (RPL5,EF1A,PICALM,TUBB,ITGB2).
- 甘-3-酸盐脱酶 (GAPDH) 基因不适合作为该物种的参考基因.
- 这项研究提供了必要的基因参考数据,以推进银尖四角动物的研究,特别是在性别分化方面.
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