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使用黑士兵 (Hermetia illucens) 水解的一种替代胺制剂:过程优化和性能评估

Gaoqiang Liu^1,2,3, Ming Foong Tiang^2,4, Shixia Ma^1,2,3

¹Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou, China.

|March 1, 2024

概括

黑兵幼虫的蛋白质可以在微生物培养介质中取代商业. 这项研究优化了水解条件,为细菌生长提供了具有成本效益的替代方案.

科学领域:

生物技术和工业微生物学
蛋白质水解和化工学
可持续的资源利用可持续的资源利用

背景情况:

黑兵 (Hermetia illucens) 被认为可以将废物转化为动物料.
黑兵幼虫 (HIL) 的高蛋白质含量提供了超出原料应用的潜力.
作为微生物培养介质中商业的替代品,对HIL蛋白的探索是有限的.

研究的目的:

为了研究使用HIL蛋白质水解盐作为子替代物的可行性.
为了优化脱脂HIL的酶解水解条件.
评估HIL在支持细菌生长中的性能.

主要方法:

使用四种商业蛋白质酶 (蛋白质酶,素,酶,帕帕因) 进行脱脂HIL的水解.
使用响应表面方法来优化水解条件 (酶度,pH,温度,时间).
细菌生长动力学 (金黄色葡萄球菌,细菌细菌,大肠杆菌) 用HIL与商业进行了分析.

主要成果:

在pH 7.0和54°C下使用1.70%重/重复酶 (性蛋白酶:三素,1:1比) 在4小时内实现最佳水解.
在最佳条件下的水解产生了19.25%±0.49%的蛋白质水解.
在HIL和商业之间没有观察到生长参数 (μmax,Xmax,λ) 的显著差异.

结论:

化酸作为一种有效和低成本的替代品,用于微生物培养商业.
这项研究证明了HIL蛋白的新型应用,将其价值扩展到动物料之外.
优化的酶性水解使昆虫蛋白质资源的有效利用成为可能.

关键词:

这是一种细菌培养.增长动力学增长动力学埃尔梅蒂亚幻灯虫幼虫的幼虫佩普是一种.

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