在Taihe黑骨不同产卵期的卵巢lncRNAs的鉴定和功能分析
Yunyan Huang1, Shibao Li1, Yuting Tan1
1College of Animal Science, Zhejiang University, Hangzhou, China.
Frontiers in physiology
|March 1, 2024
概括
长非编码RNAs (lncRNAs) 调节了泰黑骨的卵巢发育. 这项研究确定了关键的lncRNA及其向基因,这些基因涉及不同产卵期的生殖特征.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 禽畜科学 禽畜科学 禽畜科学
背景情况:
- 长非编码RNAs (lncRNAs) 是卵巢发育的关键调节者.
- 关于lncRNA在Taihe黑骨卵巢发育中的作用的研究是有限的.
- 了解lncRNA调节是改善家禽生殖特征的关键.
研究的目的:
- 为了研究 lncRNAs 在 Taihe Black-Bone 不同产卵期的卵巢发育中的作用.
- 识别差异表达的 lncRNAs (DE lncRNAs) 和它们的目标基因.
- 构建一个lncRNA-mRNA网络以阐明调控机制.
主要方法:
- 在三个产卵阶段 (早期,高峰期,晚期) 的12只泰河黑骨的卵巢组织的转录组分析 (RNA-seq).
- 识别DE lncRNA及其 cis 和 trans 目标基因.
- 构建一个lncRNA-mRNA调节网络.
主要成果:
- 在三个产卵期中确定了136个DE lncRNA.
- 发现了7个DE lncRNAs,形成14个参与生殖特征的目标基因网络.
- 目标基因与细胞因子-细胞因子受体相互作用和TGF-β信号传递等途径有关.
结论:
- lncRNAs显著影响泰黑骨的卵巢发育和生殖特征.
- 已识别的lncRNA-mRNA网络提供了有关家禽生殖调节的见解.
- 这项研究为未来关于家禽中 lncRNA 中介生殖控制的研究奠定了基础.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
02:39lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...