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通过综合性转录组分析识别喉皮状细胞癌中与fascin-actin-bundling protein 1相关的新分子和途径

Hongliang Liu¹, Wenjing Hao¹, Xinfang Wang¹

¹Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.

International journal of molecular medicine

|March 1, 2024

概括

激素活性组合蛋白1 (FSCN1) 促进喉癌的进展. 它与胆固醇生物合成中的酶24-脱胆固醇还原酶 (DHCR24) 的相互作用驱动LSCC细胞的增殖和迁移.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生化学

背景情况:

喉平细胞癌 (LSCC) 是一种流行恶性瘤,预后不佳.
素动因捆绑蛋白1 (FSCN1) 涉及到LSCC的发展,但其分子机制尚不清楚.

研究的目的:

阐明FSCN1在LSCC进展中的作用背后的分子机制.
确定涉及LSCC的新型FSCN1相互作用蛋白和途径.

主要方法:

整体转录组微阵列分析,以在FSCN1敲除后识别差异表达基因 (DEG).
同表达,蛋白质与蛋白质相互作用和交叉分析.
同免疫沉用于验证蛋白质相互作用.

主要成果:

微阵列确定了462个上调和601个下调的mRNA转录.
FSCN1与包括对病毒和类固醇生物合成的防御反应在内的功能有关.
经过验证,FSCN1与24-脱水胆固醇还原酶 (DHCR24) 相互作用,这是胆固醇生物合成中的关键酶. DHCR24过度表达增强了LSCC细胞的增殖和迁移.

结论:

DHCR24是一种与LSCC中的FSCN1相关的新型分子.
FSCN1-DHCR24的相互作用可能通过胆固醇代谢途径促进LSCC的进展.

关键词:

24-脱水胆固醇减少酶.胆固醇的代谢胆固醇的代谢共同表达的共同表达.迷人的魅力迷人的魅力喉皮状细胞癌瘤蛋白质与蛋白质的相互作用

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lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...