一种新的双Fc双特异抗体,具有增强的Fc效应器功能
Fulai Zhou1, Yinyin Ben1, Hao Jiang1
1Research & Development Department, Tavotek Biotherapeutics, Suzhou 215000, China.
Biochemistry
|March 1, 2024
概括
这项研究引入了一种新的双Fc双特异抗体 (BsAb) 格式. 这种工程抗体增强了免疫反应,为抗体基础的免疫治疗癌症和自身免疫性疾病提供了一个有前途的新选择.
科学领域:
- 免疫学 免疫学 免疫学
- 生物技术是生物技术.
- 在瘤学瘤学.
背景情况:
- 双特异性抗体 (BsAbs) 在瘤学和自身免疫性疾病治疗中至关重要.
- 目前的BsAb工程通常依赖于单个Fc参与,类似于单克隆抗体.
- 需要创新的BsAb格式,具有增强的效应器功能.
研究的目的:
- 设计和描述一种新的双Fc双特异抗体 (BsAb) 格式.
- 研究Fc工程对双Fc BsAbs.治疗疗效的影响.
- 评估双Fc BsAb结构的增强效应器功能.
主要方法:
- 设计了一种新的免疫球蛋白玛-1 (IgG1) 衍生双Fc BsAb,具有两个Fc区域和不对称的抗原结合臂 (Fab和VHH域).
- 采用了旋入孔技术,以高效生产双Fc BsAbs.
- 评估了对Fc玛受体 (FcγRs) 的结合性,并评估了抗体依赖的细胞毒性 (ADCC) 和抗体依赖的细胞化 (ADCP) 活动.
主要成果:
- 双Fc BsAb格式的产量和稳定性都很高.
- 这种新格式允许同时结合双特异抗原.
- 双Fc BsAbs表现出对FcγRs的依赖性结合增加,导致ADCC和ADCP活动增强,与单Fc对照组相比.
结论:
- 成功开发了一种具有增强效应器功能的新型双Fc双特异性抗体格式.
- 这种创新的BsAb设计为免疫治疗提供了更好的治疗潜力.
- 双Fc BsAb代表了治疗癌症和自身免疫性疾病的有希望的新策略.
相关概念视频
01:10Antibody Structure
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
01:20Special Features of Adaptive Immunity
The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
01:24B Cell Activation and Differentiation
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
01:26Antibody Actions
Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...