如何P. 如何P. 有着多样化的定子组成的细胞集体成群
Jaime de Anda1,2,3,4, Sherry L Kuchma5, Shanice S Webster5
1Department of Bioengineering, University of California Los Angeles, Los Angeles, California, USA.
mBio
|March 1, 2024
概括
Pseudomonas aeruginosa 蜂群的运动性取决于鞭毛体运动定位器 MotAB 和 MotCD. 不同的定子生产和利用,以及不同的运动活动,共同控制细菌群的转变.
科学领域:
- 微生物学 微生物学
- 细菌的运动性 细菌的运动性
- 细胞生物物理学 细胞生物物理学
背景情况:
- 在*Pseudomonas aeruginosa*中游泳是一种与表面相关的运动性.
- 旗电机由MotAB和MotCD定位器提供动力,驱动群.
- 状态器删除会以不同的方式影响群体:MotCD删除会消除它,而MotAB删除会增强它.
研究的目的:
- 研究MotAB和MotCD定位器在P. aeruginosa群中的差异性生产和利用情况.
- 了解运动间歇性和定子活动在调节从生物膜到群体过渡中的作用.
- 阐明对群群运动性和定位器选择的多因素影响.
主要方法:
- 实验测量定位器可用性和利用率.
- 在野生型和突变菌株中分析运动活动和间歇性.
- 计算机建模以了解群交的转变,并将它们与活跃颗粒物质中的干扰转变联系起来.
主要成果:
- 野生类型的P. aeruginosa表现出不对称的定位器可用性,其MotAB比MotCD产量高40倍.
- MotCD 定位器需要更高的粘度才能使用,而 MotAB 定位器在较低的粘度下工作.
- 带有MotCD定位器的电池与带有MotAB定位器的电池相比,显示了约10倍的运动活动 (间歇性).
结论:
- 群聚过渡不是由单一的参数来规范,而是通过对鞭毛活动和运动间歇性的集体控制.
- 动态定子利用和异质细胞种群有助于群体运动.
- 定位器的招募和表面传感与P. aeruginosa*中蜂群的启动和调节有关.
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