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通过在植物中表达不匹配的纠正双链RNA来控制两种昆虫害虫

Yi Dong¹, Qi Zhang¹, Yarou Mao¹

¹State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Hongshan Laboratory, School of Life Sciences, Hubei University, Wuhan, China.

Plant biotechnology journal

|March 1, 2024

概括

这项研究开发了一种新的RNA干扰 (RNAi) 方法,用于同时控制多种昆虫害虫. 转基因植物表达优化的dsRNA,保护它们免受白和虫的侵害,扩大了农业中的RNAi应用.

科学领域:

农业昆虫学农业昆虫学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

RNA干扰 (RNAi) 通过沉默重要昆虫基因,提供特定物种的害虫控制.
目前的RNAi技术仅限于针对单一的害虫物种,阻碍了广泛的农业应用.
同时控制多种昆虫害虫对于有效的作物保护至关重要.

研究的目的:

开发一种RNAi战略,同时对多种昆虫害虫进行控制.
设计和测试一种中介dSRNA (iACT),针对Bemisia tabaci和Myzus persicae中的保存基因.
评估表达毛iACT (hpiACT) 的转基因植物对这些害虫的疗效.

主要方法:

一个中间dSRNA (iACT) 的人工设计,其优化序列针对B. tabaci和M. persicae的β-Actin (ACT) 基因.
从核基因组生成表达毛iACT (hpiACT) 的转基因烟草植物.
通过评估昆虫的生育能力和目标害虫的ACT基因表达来评估植物的耐药性.

主要成果:

表达hpiACT的转基因植物单独和同时对B. tabaci和M. persicae进行了保护.
在食表达hpiACT的植物的昆虫中观察到减少生育能力和抑制ACT基因表达.
表达单目标dRNA (BtACT或MpACT) 的植物只对各自的昆虫物种产生了耐药性.

结论:

优化的dsRNA的人工合成可以通过RNAi.同时控制多种昆虫物种.
这种方法扩大了转基因RNAi技术在全面作物保护方面的实用性.
优化的dsRNA设计代表了管理各种昆虫害虫种群的新策略.

关键词:

贝米西亚烟草 (Bemisia tabaci) 是一种烟草.这种植物是Myzus persicae.这是一种RNA干扰.这是一种双链RNA.转基因植物是转基因植物.

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