瑞菌帕里迪斯氨酸通过与KEAP1结合并调节Nrf2和MAPK通路来减弱格兰氏阴性细菌诱导的炎症性
Yang Yang1,2,3, Chaofan Wang4, Juan Wang2,5
1R&D Department, Yunnan Baiyao Group Health Products Co., Ltd, Kunming, Yunnan, China.
Journal of cellular and molecular medicine
|March 1, 2024
概括
来自Rhizoma Paridis的多素 (PPH) 和其丰富的提取物 (RPE) 显示出对中涉及的格拉姆阴性细菌具有显著的抗炎和抗氧化作用. 这些发现表明潜在的新治疗方法.
科学领域:
- 皮肤病学 皮肤病学
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 是一种慢性炎症性皮肤疾病,与微生物组的改变有关.
- 阴性细菌在病原发生过程中起着重要作用.
- 需要新型的抗药物,以向阴性细菌.
研究的目的:
- 为了评估聚林 (PPH) 和PPH丰富的Rhizoma Paridis提取物 (RPE) 的抗作用.
- 阐明PPH和RPE在治疗格拉姆阴性细菌诱导的的潜在机制.
主要方法:
- 在体外研究中使用RAW 264.7和HaCaT细胞,用脂多糖 (LPS) 刺激.
- 评估了炎症性细胞因子 (IL-1β,IL-6) 的mRNA和蛋白质表达.
- 测量了细胞内活性氧物种 (ROS) 生成,NF-κB P65转位和MAPK通路活性.
- 分子对接被用来预测PPH与KEAP1的结合.
主要成果:
- PPH和RPE显著抑制了LPS诱导细胞中的IL-1β和IL-6表达和ROS生成.
- PPH和RPE抑制了NF-κB P65核转位.
- 分子对接表明PPH与KEAP1结合,导致NRF2激活和HO-1上调.
- RPE显示抑制了MAPK路径.
结论:
- 富含PPH的RPE在体外表现出强大的抗炎和抗氧化特性.
- 这些效应通过抑制炎症途径 (NF-κB,MAPK) 和激活NRF2/HO-1轴来调节.
- 富含PPH的RPE显示出作为治疗的替代治疗剂的希望.
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