相关实验视频
Updated: Jul 1, 2025

06:48
CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
6.7K
依赖SWI/SNF的基因是由它们的染色体格局定义的
Laura Basurto-Cayuela1, José A Guerrero-Martínez1, Elena Gómez-Marín1
1Genome Biology Department, Centro Andaluz de Biología Molecular y Medicina Regenerativa-CABIMER, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla-Universidad Pablo de Olavide (CSIC-USE-UPO), Av. Americo Vespucio, 41092 Seville, Spain.
Cell reports
|March 1, 2024
概括
SWI/SNF复合体维持染色质在增强剂的可访问性,而不是促进剂. 它们的活性对于具有特定促进特征的基因至关重要,揭示了染色质的特征.
科学领域:
- 染色体生物学 染色体生物学
- 基因调节 基因调节
- 分子机制的分子机制
背景情况:
- SWI/SNF复合体是依赖ATP的机器,可以重塑染色质.
- 了解它们在基因调节中的特定作用对于破译细胞过程至关重要.
研究的目的:
- 为了描述那些依赖SWI/SNF复合体的基因的特征.
- 调查SWI/SNF活动在染色质可访问性和促进剂和增强剂的核细胞占用中的作用.
主要方法:
- 使用BRM014,这是SWI/SNF ATPase活性的抑制剂.
- 分析了促进体和调节区域的染色质可访问性,核细胞占用率,组蛋白标记和基因丰富.
- 采用机器学习模型来识别SWI/SNF依赖的决定因素.
主要成果:
- 在大多数增强剂中,SWI/SNF活性对于维持染色质可访问性和核细胞占用性至关重要,但在促进剂中通常不是如此.
- SWI/SNF依赖性观察到基因的低到中表达和特定的促进体特征 (低可访问性,低活性标记,高的H3K4me1/H3K4me3比率,低分相,TATA-box丰富).
- 这些基因通常与与Brahma相关的基因1/Brahma关联因子 (BAF) 复合体,SWI/SNF依赖增强剂相关,并编码信号转导,发育或细胞身份因素.
结论:
- 促进体和周围的调节区域的染色质景观决定了SWI/SNF依赖性.
- SWI/SNF复合体在调节增强剂与促进剂方面发挥着不同的作用.
- 特定的基因类型,特别是非家政基因,依赖SWI/SNF活动通过定义的染色体特征进行适当的表达.
关键词:
这就是BRG1/SMARCA4的原因.这就是BRM/SMARCA2的原因.科普:分子生物学 分子生物学在PBAF中,PBAF是PBAF.其他替代的TSS.替代性拼接是一种替代性的拼接.反意义转录的转录cBAFFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAFBAF染色体重塑 染色体重塑 的方法核细胞的占用率 核细胞的占用率相关概念视频
Chromatin Position Affects Gene Expression
23.3K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
23.3K
Inheritance of Chromatin Structures
6.2K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.2K
Spreading of Chromatin Modifications
8.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
8.3K
Nucleosome Remodeling
9.1K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
9.1K
Chromatin Modification in iPS Cells
1.7K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.7K
Heterochromatin
12.7K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
12.7K

