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相关概念视频

Renal Corpuscle01:20

Renal Corpuscle

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The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
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The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
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Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

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Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
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Protein Folding Quality Check in the RER01:29

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ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
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Adherens Junctions

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Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
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The filtration membrane in the renal system is a highly specialized structure essential for filtering blood. It consists of glomerular capillaries and podocytes, forming a selective barrier that permits the passage of water and small solutes while restricting most plasma proteins and blood cells.
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Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
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An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney
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足细胞Ercc1对于球体完整性是不可或缺的.

Eriko Yoshida Hama1, Ran Nakamichi1, Akihito Hishikawa1

  • 1Division of Nephrology, Endocrinology and Metabolism, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.

Biochemical and biophysical research communications
|March 1, 2024
PubMed
概括

细胞ERCC1 (切割修复交叉补充组1) 对于脏健康至关重要. 它的缺乏加速衰老,并通过增加DNA损伤和炎症导致功能衰竭.

关键词:
衰老的衰老 衰老的衰老造成的DNA损伤是DNA损伤.在ERCC1的第一个阶段,ERCC1是ERCC1的成员.波多细胞 (Podocytes) 是一种细胞.

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Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation
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科学领域:

  • 腎臟病學 (nephrology) 是一種醫學專業.
  • 分子生物学分子生物学
  • 衰老研究研究 衰老研究

背景情况:

  • 预期寿命的增加导致与年龄相关的脏疾病的增加.
  • 慢性病 (CKD) 与衰老有关,可能加速衰老过程.
  • 在衰老和疾病中DNA修复的作用需要进一步调查.

研究的目的:

  • 为了研究 podocyte ERCC1 (切割修复交叉补充组1) 在保持淋巴细胞完整性方面的作用.
  • 为了探索 podocyte ERCC1 缺乏对衰老标志物和全身炎症的影响.
  • 评估ERCC1基因转移在减轻损伤方面的潜力.

主要方法:

  • 产生特定于 podocyte 的 ERCC1 淘汰赛小鼠.
  • 分析功能,球组织学和DNA损伤标记 (SSB和DSB).
  • 对T细胞衰老 (CD44+CD8+记忆T细胞) 和与衰老相关的分泌表型 (SASP) 因素的评估.
  • 在老年小鼠和人类脏样本中评估ERCC1表达和DNA损伤.

主要成果:

  • 足细胞ERCC1的淘汰导致严重的蛋白尿症,血球硬化,功能衰竭,以及增加的血球细胞DNA断裂.
  • 转移ERCC1基因改善了蛋白尿和血球样硬化,减少了DNA损伤.
  • 由于ERCC1缺乏,T细胞衰老,系统性和多个器官的SASP因子升高.
  • 在老年小鼠和人类患者的质体中观察到DNA断裂的积累,特别是SSB.

结论:

  • 足细胞ERCC1对于修复DNA损伤和维持质细胞结构至关重要.
  • 细胞中ERCC1缺乏会加速老化表型,包括T细胞衰老和全身炎症.
  • 向ERCC1可能为与年龄相关的脏疾病和相关炎症提供治疗潜力.