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针对性下一代测序试验用于直接检测和从缩中测定沙门氏菌的血清型

Andrew Lin¹, Atul Singh¹, Adam Allred¹

¹Clear Labs, Inc, 1559 Industrial Rd, San Carlos, CA 94070, USA.

Journal of food protection

|March 1, 2024

概括

一种自动定向下一代测序 (tNGS) 试验能够准确地检测和测定家禽样本中的沙门氏菌的血清型. 这种方法显著减少了检测时间从几天到几个小时,提高了食品安全监督.

科学领域:

微生物学微生物学
基因组学就是基因组学.
食品安全食品安全

背景情况:

沙门氏菌污染对家禽生产和公共健康构成重大风险.
传统的沙门氏菌检测和血清定型方法耗时且劳动密集.

研究的目的:

为了评估自动化,有针对性的下一代测序 (tNGS) 试验对沙门氏菌检测和血清型定型的性能.
与传统方法相比,评估tNGS的准确性和效率.

主要方法:

该研究评估了使用纯种植物,人工污染样本和来自家禽设施的自然污染环境样本的自动化tNGS试验.
对291种纯基准培养和各种家禽相关样本类型进行了准确性测试.
此外,还评估了共同污染场景中的检测和性能极限.

主要成果:

tNGS测定在检测沙门氏菌时达到100%的准确性,在从纯种植物中测定62种常见的沙门氏菌血清型时达到100%的准确性.
在纯培养试验中没有观察到假阳性.
该试验在人工污染的家禽样本中显示出100%的准确性,并且在环境样本中有效检测沙门氏菌,在12-12小时后获得结果.

结论:

自动化tNGS测定提供了一种可靠和有效的方法,用于在复杂矩阵中检测沙门氏菌和血清类型.
与传统方法相比,这项技术大大缩短了交付时间,为家禽产业监督和食品安全提供了宝贵的工具.
tNGS检测的高精度和速度支持其用于快速监测沙门氏菌的实施.

关键词:

完全自动化的全自动化.沙门氏菌是一种沙门氏菌.血清类型的确定.有针对性的下一代测序.

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