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相关概念视频

Atomic Force Microscopy01:08

Atomic Force Microscopy

3.4K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Studying the Cytoskeleton01:17

Studying the Cytoskeleton

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The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
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相关实验视频

Updated: Jul 1, 2025

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy
11:10

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy

Published on: August 28, 2011

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使用原子力显微镜分析原生肺底膜刚度.

Bastian Hartmann1,2, Lutz Fleischhauer1,2, Monica Nicolau3,4

  • 1Munich University of Applied Sciences, Center for Applied Tissue Engineering and Regenerative Medicine - CANTER, Munich, Germany.

Nature protocols
|March 1, 2024
PubMed
概括
此摘要是机器生成的。

这项研究介绍了一项测量肺弹性模块的协议.

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Extracting the Young's Modulus of Native Murine Pulmonary Basement Membranes from Atomic Force Microscopy Derived Force Maps
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Extracting the Young's Modulus of Native Murine Pulmonary Basement Membranes from Atomic Force Microscopy Derived Force Maps

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Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy
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Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy

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相关实验视频

Last Updated: Jul 1, 2025

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11:10

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy

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Extracting the Young's Modulus of Native Murine Pulmonary Basement Membranes from Atomic Force Microscopy Derived Force Maps
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Extracting the Young's Modulus of Native Murine Pulmonary Basement Membranes from Atomic Force Microscopy Derived Force Maps

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Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy
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Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy

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科学领域:

  • 生物物理学的生物物理.
  • 细胞生物学 细胞生物学
  • 材料科学 材料科学 材料科学

背景情况:

  • 哺乳动物细胞对微环境机制做出反应.
  • 底层膜 (BM) 生物力学对瘤进展至关重要.
  • 描述像BM这样的薄型ECM结构是具有挑战性的.

研究的目的:

  • 开发一种用于量化肺底膜 (BM) 弹性模块的协议.
  • 为了实现肺组织的高分辨率生物力学表征.

主要方法:

  • 鼠类和人类肺部组织提取,冷解剖和原子力显微镜 (AFM) 力图记录的协议.
  • 使用自定义的AFM软件进行半自动数据分析,用于BM识别和Young的模量计算.
  • 用于空间生物力学分析的模块图的生成.

主要成果:

  • 为BM弹性模量定量建立了一个详细,精简的协议.
  • 该协议允许半自动识别和分析肺部BM.
  • 可以生成BM的Young的模量图.

结论:

  • 该协议为肺组织中的BM生物力学提供了宝贵的见解.
  • BM的高分辨率生物力学数据具有转移的诊断潜力.
  • 该方法为研究健康和疾病中的BM特性提供了一种可行的方法.