使用基于TMT的定量蛋白质学方法,对急性心肌梗塞后心脏破裂患者的血蛋白质进行蛋白质学分析
Jingyuan Hou1,2, Qiaoting Deng1, Xiaohong Qiu3
1Research Experimental Center, Meizhou Clinical Institute of Shantou University Medical College, Meizhou, Guangdong, 514031, China.
Clinical proteomics
|March 1, 2024
概括
这项研究确定了心脏破裂 (CR) 患者的关键血蛋白变化,揭示了CRP,HSPB1,VINC和GDF15等潜在生物标志物,以改善急性心肌梗塞 (AMI) 后的诊断.
科学领域:
- 蛋白质组学是指蛋白质组学.
- 心血管医学 心血管医学
- 生物标志物发现发现
背景情况:
- 心脏破裂 (CR) 是急性心肌梗塞 (AMI) 的罕见但致命的并发症.
- 可靠的诊断生物标志物和CR的潜在信号通路仍然不清楚.
- 了解CR机制对于改善患者治疗结果至关重要.
研究的目的:
- 在心脏破裂患者中全面描述血蛋白质组变化.
- 为了确定潜在的蛋白质生物标志物用于CR的临床诊断.
- 为了深入了解参与CR病变的分子途径.
主要方法:
- 使用双重质量标签 (TMT) 标签和液体染色学-双重质量谱学 (LC-MS/MS) 的定量蛋白质组学.
- 分析了CR (n=37),AMI (n=47) 和健康对照组 (n=47) 的患者的血样本.
- 使用多重反应监测 (MRM) 和酶相关免疫吸收试验 (ELISA) 验证候选蛋白质.
主要成果:
- 量化了1208种蛋白质,确定了958种差异表达蛋白质 (DEP).
- 与对照组和AMI患者相比,CR患者的DEP显著改变.
- 生物信息学分析涉及的途径包括RNA运输,亡和白细胞在CR中的超内皮细胞迁移.
- 通过ELISA验证的四种蛋白质 (CRP,HSPB1,VINC,GDF15) 显示出高诊断精度 (AUC=0.895) 来区分CR与AMI.
结论:
- 综合蛋白质组分析对于识别CR中的血蛋白质组变化是有价值的.
- 这项研究为阐明CR机制和开发诊断生物标志物提供了基础.
- 鉴定的蛋白质面板有望帮助早期诊断心脏破裂.
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