相关实验视频

Updated: Jul 1, 2025

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

血清蛋白质组分析确定了轴性牛皮关节炎的潜在生物标志物

Chaofan Lu¹, Fan Yang¹, Shihao He¹

¹Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, National Clinical Research Center for Dermatologic and Immunologic Diseases (NCRC-DID), Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, No1 Shuaifuyuan Wangfujing, Dongcheng District, Beijing, 100730, China.

European journal of medical research

|March 1, 2024

概括

研究人员确定了色素表皮衍生因子 (PEDF) 作为潜在的血清生物标志物,以区分轴性牛皮关节炎 (axPsA) 和外围性牛皮关节炎 (pPsA),有助于早期诊断.

科学领域:

风湿病学和免疫学
蛋白质组学和生物标志物发现发现

背景情况:

牛皮关节炎 (PsA) 呈现出明显的轴向性 (axPsA) 和外周性 (pPsA) 疾病模式.
精确区分axPsA和pPsA对于有针对性的治疗策略至关重要.
当前的诊断方法可能并不总能清楚地区分这些PsA亚型.

研究的目的:

识别和验证能够区分轴性牛皮关节炎 (axPsA) 和外围性牛皮关节炎 (pPsA) 的血清生物标志物.
为了研究axPsA和pPsA患者队列之间的蛋白质差异.
评估 axPsA.所识别的生物标志物的诊断潜力.

主要方法:

在发现队列中,使用非向蛋白质组学分析了血清样本.
候选生物标志物在单独的验证队列中使用酶相关免疫吸收试验 (ELISA) 进行了验证.
使用了统计分析,包括Boruta算法,拉索回归和接收器运行特征 (ROC) 分析.

主要成果:

在axPsA和pPsA之间确定了45种不同表达的蛋白质.
血清色素表皮衍生因子 (PEDF) 被确定为一个关键的差异化生物标志物.
与pPsA患者相比,axPsA患者的血清PEDF升高水平显著高于pPsA患者 (p < 0.001),AUC为0.72.

结论:

这项研究提供了详细的蛋白质组特征,区分axPsA和pPsA.
血清PEDF成为早期诊断axPsA的有希望的候选生物标志物.
进一步的研究可能会探索PEDF在疾病活动中的作用及其治疗含义.

关键词:

关节炎,牛皮,牛皮.轴向的PSA是一个PSA.生物标志物生物标志物这是LC-MS/MS.蛋白质组是蛋白质组的组成部分.

更多相关视频

Author Spotlight: Self-Assessment Protocol for Predicting Psoriatic Arthritis in Psoriasis Patients

Author Spotlight: Self-Assessment Protocol for Predicting Psoriatic Arthritis in Psoriasis Patients

Published on: March 1, 2024

Synovial Fluid Analysis to Identify Osteoarthritis

Synovial Fluid Analysis to Identify Osteoarthritis

Published on: October 20, 2022

相关概念视频

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...