多omics数据阐明了寄生虫-宿主-微生物群相互作用和在绵羊中对Haemonchus contortus的耐药性
Simone Cristina Méo Niciura1, Tainã Figueiredo Cardoso2, Adriana Mercia Guaratini Ibelli2
1Embrapa Pecuária Sudeste, São Carlos, SP, Brazil. simone.niciura@embrapa.br.
Parasites & vectors
|March 1, 2024
概括
这项研究揭示了关键的基因和途径,涉及到羊对Haemonchus contortus寄生虫的抵抗力. 这些发现有助于培育耐寄生虫的绵羊,并制定针对这一主要牲畜威胁的新控制策略.
科学领域:
- 动物基因组学 动物基因组学
- 寄生虫学的寄生虫学
- 微生物组研究 微生物组研究
背景情况:
- 海蒙丘斯 (Haemonchus contortus) 在羊群中造成重大经济损失,而这种损失因抗甲虫药物耐药性而加剧.
- 了解宿主-寄生虫-微生物群相互作用对于改善绵羊的寄生虫耐药性至关重要.
- 培育耐药性和开发新疗法对于长期控制虫至关重要.
研究的目的:
- 确定分子因素,包括宿主基因和微生物群落,与绵羊对Haemonchus contortus感染的耐药性相关.
- 探索羊宿主,其胃肠道微生物群和寄生虫之间的复杂相互作用.
- 为基因选择策略和对H.contortus.的新型控制方法提供基础.
主要方法:
- 羊腹部和H. contortus寄生虫的转录组测序 (RNA-seq).
- 单核酸多态化 (SNP) 羊的基因定型.
- 16S核糖体RNA基因测序用于在绵羊便和牛中进行微生物群体分析 (ASV).
主要成果:
- 寄生虫卵数 (EPG) 的遗传率估计为0.12.
- 在耐药与易感羊群中,差异性基因表达确定了GAST,GNLY,IL13,MGRN1,FGF14和RORC.
- 全基因组关联研究确定了染色体2和11上的耐药性相关区域.
- 微生物群分析显示了特定细菌属 (Christensenellaceae, Bacteroides, Methanobrevibacter) 和寄生虫耐药性之间的相关性.
- 寄生虫基因表达在耐药和易受宿主感染之间有所不同.
结论:
- 确定了与绵羊寄生虫耐药性相关的特定基因,转录和基因组区域.
- 突出了宿主微生物群相互作用在调节对H. contortus.免疫反应中的作用.
- 这些发现支持对抗寄生虫的绵羊和综合控制方法的选择策略的开发.
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