该circRNA hsa-circ-0013561通过miR-7-5p/PDK3轴调节头部和部状细胞癌的发展
Kaisai Tian1,2, Liying Zheng1,2, Tailei Yuan1
1Postgraduate Training Base at Shanghai Gongli Hospital, Ningxia Medical University, Shanghai, 200135, China.
Cancer cell international
|March 1, 2024
概括
低调 hsa-circ-0013561 抑制了头部和部状细胞癌 (HNSCC) 的进展和转移. 这通过调节miR-7-5p结合和PDK3表达而发生,影响细胞增殖和迁移.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 循环RNAs (circRNAs) 与癌症进展有关.
- Hsa-circ-0013561异常表达在头部和部状细胞癌 (HNSCC) 中.
- 在HNSCC进展中hsa-circ-0013561的确切作用尚不清楚.
研究的目的:
- 研究hsa-circ-0013561在HNSCC进展和转移中的作用.
- 在HNSCC中确定hsa-circ-0013561的下游目标和监管机制.
主要方法:
- 光在位杂交 (FISH) 和qRT-PCR用于评估hsa-circ-0013561表达.
- 双路西法酶记者测定确定了下游目标.
- 跨井迁移,EDU,CCK8和殖民地形成试验评估了细胞行为.
- 一只老鼠异种移植模型评估了瘤进展和转移的体内影响.
主要成果:
- 发现Hsa-circ-0013561在HNSCC组织中受到上调.
- 下调hsa-circ-0013561抑制了HNSCC细胞的增殖和迁移,促进了细胞亡和G1细胞周期的停止.
- 确定了miR-7-5p和PDK3作为下游目标.
- 过度表达PDK3或抑制miR-7-5p可以逆转hsa-circ-0013561下调的抑制作用.
结论:
- 低调 Hsa-circ-0013561 抑制 HNSCC 的转移和进展.
- 该机制涉及调节PDK3表达和miR-7-5p结合.
- 这项研究阐明了HNSCC.的新型监管途径.
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