使用生物信息学和机器学习识别MASH和DN中ECM受体相互作用基因的交叉基因
Chao Chen1, Yuxi He2, Ying Ni3,4
1Instrumentation and Service Center for Science and Technology, Beijing Normal University, Zhuhai, China.
Journal of cellular and molecular medicine
|March 2, 2024
概括
研究人员确定了代谢功能障碍相关的脂肪肝疾病 (MASH) 和糖尿病病 (DN) 之间共享的关键基因. 一个涉及巨细胞和IL-17通路的FOS/FOXC1/HMMR轴可能将这些条件联系起来,并提供治疗点.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 病理学 病理学 病理学
背景情况:
- 与代谢功能障碍相关的脂肪肝病 (MASH) 和糖尿病病 (DN) 是重叠病理的重大健康问题.
- 了解共享的遗传因素和分子途径对于开发有效的治疗方法至关重要.
研究的目的:
- 为了确定MASH和DN之间的共同基因.
- 研究细胞外矩阵 (ECM) 受体相互作用基因在这些共享途径中的作用.
- 阐明潜在的监管轴和治疗目标.
主要方法:
- 从GEO数据库下载了MASH和DN基因表达数据集.
- 分析了基因相关性,共同表达网络和蛋白质-蛋白质相互作用.
- 利用机器学习 (XGBoost) 来进行基本的基因识别.
- 使用CIBERSORT进行了免疫透分析.
主要成果:
- 确定了19个MASH和DN的共同关键联合表达对 (CP) 基因.
- 原瘤基因 (FOS) 在PPI网络中具有很高的中心作用,并且与IL-17通路有关.
- 在共同表达网络中,氨酸介导运动受体 (HMMR) 是关键的.
- 叉头盒 C1 (FOXC1) 显示出强大的疾病预测能力.
- FOS和FOXC1与参与IL-17分泌的乳腺细胞正相关.
结论:
- 一个FOS/FOXC1/HMMR调控轴,可能涉及巨细胞和IL-17信号通路,被建议用于MASH和DN.
- 细胞外HMMR可以充当信号载体,通过ERK或PI3K-Akt-mTOR通路调节FOS代表的IL-17通路.
- 对于MASH和DN来说,HMMR是一个潜在的治疗标.
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