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miR-378a-5p通过准TSPAN8抑制了巴雷特食道细胞的增殖,迁移和入侵

Xudong Xie¹, Duo Wei²

¹Department of Gastroenterology Ward 2, Xianyang Hospital of Yan'an University, Xianyang, Shaanxi 712000, China. xiexudong999@163.com.

Cellular and molecular biology (Noisy-le-Grand, France)

|March 2, 2024

概括

微RNA-378a-5p (miR-378a-5p) 通过向TSPAN8.8来抑制巴雷特食道 (BE) 的进展. 降低的miR-378a-5p和升高的TSPAN8促进BE细胞的增殖,迁移和入侵.

科学领域:

胃肠病学胃肠病学
分子生物学分子生物学
在瘤学瘤学.

背景情况:

巴雷特食道 (Barrett's esophagus,BE) 是一种食道的病理状况.
推动BE进展的分子机制需要进一步阐明.
微RNAs (miRNAs) 在细胞过程和疾病发展中起着至关重要的作用.

研究的目的:

研究miR-378a-5p及其标TSPAN8在巴雷特食道进展中的功能作用.
探索针对miR-378a-5p/TSPAN8轴在BE治疗干预的潜力.

主要方法:

GEO数据集分析 (GSE26886) 以确定差异表达的基因.
试验室细胞模型 (CP-A,CP-B细胞) 暴露于胆酸 (BA) 以模仿BE条件.
定量逆转录PCR (RT-qPCR) 和西班牙涂抹来评估基因和蛋白质表达.
细胞计数工具-8 (CCK-8),伤口划伤和Transwell测试用于评估细胞增殖,迁移和入侵.
相关性分析,双露西法酶记者测定和救援实验以确认目标关系.

主要成果:

在BE样本中,TSPAN8被确定为高表达基因.
在接受BA治疗的BE细胞中,miR-378a-5p的表达减少,而TSPAN8的表达增加.
降低TSPAN8的调节抑制了BA诱导的BE细胞的增殖,迁移和入侵.
miR-378a-5p通过准TSPAN8.8来抑制BE细胞的增殖,迁移和入侵.
miR-378a-5p/TSPAN8相互作用影响CDX2和SOX9的表达,可能导致BE的形成.

结论:

miR-378a-5p作为巴雷特食道的瘤抑制剂,通过TSPAN8向抑制细胞增殖,迁移和入侵.
miR-378a-5p/TSPAN8轴代表了管理BE进展的潜在治疗目标.
对miR-378a-5p和TSPAN8的失调会影响关键的转录因子 (CDX2,SOX9),从而导致BE的发病.

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