益生菌Lactobacillus rhamnosus的X射线结构和特征 益生菌 益生菌-M9 L-rhamnose异构酶
Hiromi Yoshida1,2, Naho Yamamoto3, Lin Hai Kurahara4
1Department of Basic Life Science, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-Cho, Kita-Gun, Kagawa, 761-0793, Japan. yoshida.hiromi@kagawa-u.ac.jp.
Applied microbiology and biotechnology
|March 2, 2024
概括
这项研究报告了来自Lactobacillus rhamnosus Probio-M9.的新型L-rhamnose异构酶 (L-RhI) 的晶体结构. 在酸性条件下,该酶对L-rhamnose和D-allulose等罕见糖具有活性,为罕见糖的生产提供了潜力.
科学领域:
- 生物化学和酶学 生物化学和酶学
- 结构生物学 结构生物学
- 微生物学 微生物学
背景情况:
- 激素L-rhamnose异构酶 (L-RhI) 对于L-rhamnose的代谢至关重要.
- 一些L-RhI可以异构稀有糖 (D-氨酸,D-氨酸),使它们对生产有价值.
- 已知L-RhI的有限的晶体结构和基本的pH最佳值阻碍了它们的应用.
研究的目的:
- 为了确定L. rhamnosus Probio-M9 L-RhI (LrL-RhI) 与其基质的复合体中的晶体结构.
- 研究该酶的活性概况,特别是在酸性条件下对稀有糖进行研究.
- 为酶工程提供对基质识别和催化机制的结构洞察力.
主要方法:
- 再组合L. rhamnosus的表达和净化 益生菌-M9 L-RhI (LrL-RhI).
- 结晶和结晶结构的确定与L-rhamnose,D-allulose和D-allose复合物的结晶.
- 在酸性条件下对酶活性进行测定.
主要成果:
- 晶体结构显示LrL-RhI与L-rhamnose,D-allulose (包括环开形式) 和D-allose复合在一起.
- 在酸性条件下,LrL-RhI表现出对L-rhamnose和D-allulose的酶活性,以及对D-allulose的低活性.
- 结构分析表明,对L-糖的催化和D-糖的潜在抑制有有利的认识.
结论:
- LrL-RhI 的晶体结构为其基质特异性和催化活性提供了分子基础.
- 由于LrL-RhI在酸性条件下和对稀少糖的活性,使其成为工业应用的有希望的候选者.
- 结构洞察力可以指导未来的酶工程工作,以优化罕见糖的生产.
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