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亚克雷莫尼亚 sp. 亚克雷莫尼亚 sp. 滴糖酶-助力化学品的多样化:产业副产品的价值化

Micaela Baglioni¹, Alexander Fries¹, Jan-Mathis Müller^1,2

¹INCITAP-CONICET, FCEyN-Universidad Nacional de La Pampa (UNLPam), Av. Uruguay, 151, Santa Rosa, La Pampa, Argentina.

Applied microbiology and biotechnology

|March 2, 2024

概括

这项研究表明,使用真菌滴糖酶α-rhamnosyl-β-glucosidase I (αRβG I) 来从类废物中合成有价值的规律化物. 生物催化工艺高效地将hesperidin转化为有用的化合物,如糖素.

科学领域:

生物催化剂和酶技术
绿色化学和可持续的过程
生物炼油厂和废物回收利用

背景情况:

果加工产生了大量的农业副产品,通常富含有价值的化合物,如素.
菌滴糖酶为天然产品的酶改造提供了潜在的潜力.
农业废弃物和低成本基质如糖醇的价值化对可持续工业至关重要.

研究的目的:

为了探索真菌α-rhamnosyl-β-glucosidase I (αRβG I) 在合成rutinosides中的应用.
开发一种使用树皮残留物作为素来源的单生物催化工艺.
调查从甘油和素中有效合成和净化甘素 rutinoside.

主要方法:

一生物催化剂使用来自Acremonium sp.的αRβG I. 在 DSM 24697.7.
利用类树皮残留物作为糖化反应的hesperidin供体.
采用甘油醇作为合成甘油素rutinoside的受体.
使用活性炭和液体-液体提取来净化甘鲁丁酸,以回收赫斯佩雷丁.

主要成果:

成功合成了4 - 甲基贝利费里尔鲁丁oside (4-MUR) 和糖鲁丁oside.
在高糖化/水解选择性下,实现了99%的甘鲁丁化合成产量.
作为副产品的hesperetin被证明是有效的回收.
验证了αRβG I在农业副产品和糖的价值化中的使用.

结论:

开发的生物催化工艺为生产 rutinosides 提供了一种可持续的方法.
αRβG I在提升果加工残留物和甘油的价值方面发挥着关键作用.
这种方法为农业废物管理和增值提供了一个有前途的生物炼油战略.

关键词:

糖基化/水解选择性的选择性多种酒精多种酒精基质特异性基质特异性经过糖基化转化.

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