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完整的基因组组装提供了一个高质量的骨架,用于在瓜子中进行泛NLRome构建

Changjuan Mo¹, Haiyan Wang¹, Minghua Wei¹

¹National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.

The Plant journal : for cell and molecular biology

|March 2, 2024

概括

野生西瓜的胚胎质提供了尚未开发的疾病耐药性的遗传资源. 一个完整的基因组组装和泛NLRome揭示了对西瓜的洞察力.

科学领域:

植物基因组学植物基因组学
作物遗传学作物遗传学
分子生物学分子生物学

背景情况:

瓜子 (Cucumis melo L.) 由于化和繁殖而表现出显著的表型多样性.
野生西瓜的胚胎质中含有宝贵的遗传资源,可以耐受压力和抵抗疾病.
了解瓜子基因组架构对于改善作物特征至关重要.

研究的目的:

创建一个高质量的,没有差距的野生西瓜加入PI511890.0.的基因组组合.
为了表征耐药性基因类似物,特别是核酸结合性白丰富的重复受体 (NLRs),在西瓜.
为瓜子育种和疾病耐药性研究提供基因组资源.

主要方法:

野生西瓜的端粒到端粒 (T2T) 和无间隙基因组测序PI511890.
使用七个西瓜基因组构建一个泛NLRome.
对NLR基因表达的转录组分析,以应对病原体诱导.

主要成果:

生成了野生西瓜的完整T2T基因组组合 (375.0 Mb) 的N50密度为31.24 Mb.
全NLRome在七个西瓜基因组中确定了208个可变和18个核心核酸结合氨酸丰富的重复受体 (NLR).
转录组数据显示,外和云NLR在病原体挑战时主要受到上调.

结论:

T2T无间隙组合和泛NLRome是瓜子基因组研究和分子育种的宝贵资源.
这些资源提供了关于西瓜基因组架构和NLR多样性的见解.
这些发现有助于在瓜子育种计划中发现和利用抗病基因.

关键词:

没有空隙的组件组合.瓜子瓜子瓜子全国NL罗马罗马耐药性基因类似物端粒到端粒的时间.

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相关概念视频

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Genomics

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...