长非编码RNA PWRN1通过调节粒粉细胞的功能来影响卵巢卵泡的发育
Tongwei Zhang1, Junya Zhang1, Guang Yang1
1Center for Reproductive Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Henan Key Laboratory of Reproduction and Genetics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Reproductive biomedicine online
|March 2, 2024
概括
普拉德-威利区域非蛋白质编码RNA 1 (PWRN1) 对卵巢功能至关重要. 较低的PWRN1水平会损害颗粒细胞功能和卵泡发育,影响生育能力.
科学领域:
- 生殖生物学 生殖生物学
- 分子内分泌学分子内分泌学
- 遗传学 是一个遗传学.
背景情况:
- 卵巢卵泡的发育对于女性生育至关重要.
- 非编码RNA在卵巢功能中的作用是一个新兴的研究领域.
- 卵巢储备减少 (DOR) 是不孕症的一个重要原因.
研究的目的:
- 研究普拉德-威利区域非蛋白质编码RNA 1 (PWRN1) 在卵巢卵泡发育中的作用.
- 阐明PWRN1在颗粒细胞中的功能背后的分子机制.
- 在体内评估PWRN1对卵巢功能的影响.
主要方法:
- 检测到PWRN1表达在来自不同卵巢功能的患者的人类颗粒细胞中.
- 用于体外细胞培养 (初级颗粒细胞,KGN细胞系) 来研究PWRN1对细胞功能的影响.
- 采用了体外 (新生小鼠卵巢培养) 和体内 (小鼠模型) 实验来评估PWRN1对卵巢功能的影响.
主要成果:
- 在患有DOR.患者的颗粒细胞中,PWRN1的表达显著降低.
- 降低PWRN1的调节促进了颗粒状细胞的亡,细胞循环停止,自的增加和降低了类固醇生成能力.
- 在体内,减少PWRN1损害了卵泡发育,影响了雌性周期,并减少了小鼠的卵细胞和胚胎产量.
结论:
- lncRNA PWRN1在调节颗粒细胞功能和卵巢卵泡发育方面发挥着至关重要的作用.
- PWRN1的失调与卵巢储备的减少有关.
- PWRN1代表了改善卵巢功能和生育能力的潜在治疗标.
相关概念视频
01:20Folliculogenesis
765
Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...
765
01:30Hormonal Control of the Ovarian Cycle
496
The ovarian cycle is meticulously regulated by the hypothalamic-pituitary-gonadal axis. This cycle orchestrates the release of a mature oocyte, essential for reproduction.
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
496
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
02:55The Nucleolus
8.8K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.8K
01:22Oogenesis
1.5K
Oogenesis, the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
1.5K
02:57piRNA - Piwi-interacting RNAs
6.9K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.9K