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基于氨酸的水凝具有可调节的机制,改善了细胞的结构和收缩特性

Šimon Klimovič¹, Deborah Beckerová², Jakub Věžník³

¹CEITEC, Masaryk University, Brno, Czech Republic; Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.

Biomaterials advances

|March 2, 2024

概括

这项研究引入了由氨酸 (HA) 和凝制成的可调节的水凝,模仿细胞外基质 (ECM),以获得更好的细胞培养. 这些生物相容材料支持细胞粘附,并在二维和三维培养中改善细胞功能.

科学领域:

生物材料科学生物材料科学
细胞生物学细胞生物学
组织工程是组织工程.

背景情况:

细胞外基质 (ECM) 对于通过机械传导进行细胞反应至关重要.
在塑料表面的标准体外细胞培养忽略了ECM的机械影响.
对于支持细胞粘附和增殖的2D/3D细胞培养,需要具有成本效益的,可调节的生物材料.

研究的目的:

开发一种可调节的水凝系统,使用氨酸 (HA),牛血清白蛋白 (BSA) 和凝.
研究这些水凝的机械特性,结构特征和细胞相互作用.
评估水凝对二维和三维细胞培养的适用性及其对细胞行为和功能的影响.

主要方法:

通过使用EDC/NHS进行高分子量HA,BSA和凝的交叉链接来合成可调节的水凝.
通过不同的HA和BSA度来描述水凝的机械性能 (Young的模量).
评估水凝的结构性质,包括集群大小和膨胀能力.
评估的介质和氧气扩散对3D培养的适用性.
在水凝上培养的小鼠胚胎纤维细胞 (MEF) 的机械和结构变化与塑料相比较.
用HL-1细胞系测试水凝性能,以评估机械和电生理学特性.

主要成果:

通过从亚千帕斯卡到千帕斯卡的扬斯模块实现了可调节的水凝.
较高的HA和BSA度导致更硬的水凝,集群大小和胀减少.
水凝显示出足够的介质和氧气扩散,适合3D细胞培养.
在水凝上培养MEF诱导了actin丝结构的变化,表明有效的机械传导.
水凝促进了细胞的粘附和增殖.
使用HL-1细胞的应用显示了增强的机械和电生理学收缩性质.

结论:

开发的HA-BSA-凝水凝为细胞培养提供可调节的机械和结构性质.
这些水凝有效地模仿自然ECM的各个方面,促进细胞粘附和改变细胞结构.
该系统适用于二维和三维细胞培养,对先进的基于细胞的测试和组织工程应用有前途.
水凝改善了细胞的机械和电生理功能,特别是在复杂的细胞系中.

关键词:

航空飞行管理 (AFM)动蛋白结构动蛋白结构在 HL-1 的情况下,它是 HL-1.整体图形 (holotomography) 是一个全息图形.氨酸是什么? 氨酸是什么?水凝是一种水凝.在MEA中,MEA是MEA.机械转导是指机械转导的过程.可调节的属性调节的属性

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