CsWRKY21的替代拼接积极调节茶叶植物的冷反应
Xiaozeng Mi1, Mengsha Tang1, Jiaxin Zhu2
1State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, West 130 Changjiang Road, Hefei, Anhui, 230036, People's Republic of China; Guizhou Tea Research Institute, 1 Jin'nong Road, Guiyang, Guizhou, 550006, People's Republic of China.
Plant physiology and biochemistry : PPB
|March 2, 2024
概括
CsWRKY21基因的替代拼接增强了茶叶植物的寒冷耐受性. 这一过程增加了CsWRKY21蛋白水平,在低温下调节了酸含量.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物应激生理学 植物应激生理学
- 转录后的监管 转录后的监管
背景情况:
- 替代拼接 (AS) 对于植物的抗压能力至关重要.
- WRKY转录因子调节植物的发育和应激反应.
- 茶叶植物对寒冷耐受性的 WRKY AS 的作用在很大程度上是未知的.
研究的目的:
- 研究CsWRKY21替代拼接在茶叶植物耐寒性中的功能.
- 阐明CsWRKY21介导的冷反应背后的分子机制.
主要方法:
- 基因表达分析 (RT-qPCR)
- 亚细胞局部化检测试验
- 转录活动测定检测.
- 酵母单杂交 (Y1H) 和双化酶记者测定.
- 西方斑点分析分析
主要成果:
- CsWRKY21表达是由低温诱导的.
- CsWRKY21作为一个转录抑制剂,抑制CsABA8H和CsUGT.
- 在5'UTR中CsWRKY21的替代拼接会产生CsWRKY21-b,它通过冷调高调节.
- 在CsWRKY21-b中的内保留增加了CsWRKY21蛋白水平,降低了酸 (ABA) 含量.
结论:
- CsWRKY21的替代拼接是茶叶植物耐寒的一个关键机制.
- CsWRKY21的冷诱导AS调节蛋白质水平和ABA生物合成.
- 这项研究提供了关于植物中AS介导的低温防御机制的见解.
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