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DNA多面体立方体,镜和方形金字塔保护着催化酶的催化活性:热力学和动力学研究

Kajal Sundaray¹, Bineeth Baral¹, Umakanta Subudhi¹

¹DNA Nanotechnology & Application Laboratory, Environment and Sustainability Department, CSIR-Institute of Minerals & Materials Technology, Bhubaneswar 751013, Odisha, India; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad 201002, India.

International journal of biological macromolecules

|March 2, 2024

概括

DNA多面体保护抗氧化酶催化酶免于展开,证明生物相容性. 这些纳米结构显示出细胞应用的希望,因为它们的保护作用和缺乏毒性.

科学领域:

纳米技术和分子生物学
生物化学和生物物理学

背景情况:

DNA纳米结构,特别是DNA多面体,是创建3D纳米结构的多功能构建块.
对DNA多面体的细胞相互作用和生物影响的研究是有限的.

研究的目的:

为了研究DNA多面体和抗氧化酶催化酶之间的分子相互作用.
评估DNA多面体对触酶酶活性,构造和细胞生物相容性的影响.

主要方法:

酶活性测定是在用DNA多面体化牛肝催化酶 (BLC) 上进行的.
用光谱学研究 (光火) 来分析蛋白质构成和结合相互作用.
动力学研究和热力学参数分析阐明了相互作用机制.
细胞毒性测试评估了DNA多面体在细胞环境中的生物相容性.

主要成果:

DNA多面体保护了BLC活动在24小时化后不展开.
动力分析揭示了DNA多面体在BLC上的保护作用,由较低的K_M和更高的催化效率表明.
谱学研究表明,在与DNA多面体相互作用时,BLC中没有显著的构造变化.
光火和热力学数据证实了DNA多面体和BLC之间的非共价结合.
肝酶活性保持不变,细胞毒性测定证实了DNA多面体的生物相容性.

结论:

DNA多面体对酶酶活性表现出保护作用,而不会引起显著的形状变化.
DNA多面体和触酶之间的非共价相互作用有助于酶稳定.
DNA多面体表现出极好的生物相容性,适合在细胞环境中使用.

关键词:

催化酶是一种催化剂.立方体的立方体在DNA纳米结构中.它们是DNA多面体.酶动力学酶动力学

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