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新确诊结核病患者的口腔微生物组;一个试点研究
Muhammad Shahzad1, Muhammad Saeed2, Humaira Amin3
1Faculty of Dentistry, Zarqa University, Zarqa 13110, Jordan; Institute of Basic Medical Sciences, Khyber Medical University, Hayat Abad Phase 5, Peshawar 25120, Pakistan.
结核病 (TB) 患者表现出明显的口腔微生物变化,包括改变的多样性和 Streptococcus 等特定的细菌丰富. 这些发现表明,口腔细菌失生症在结核病发病过程中起着作用.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 口腔微生物群失生症与各种疾病有关.
- 之前没有研究研究了人类结核病 (TB) 中的口腔微生物群变化.
研究的目的:
- 为了研究新诊断的,以前没有接受过治疗的结核病患者的口腔微生物失生症.
- 为了比较结核病患者和健康对照者的口腔微生物群.
主要方法:
- 从20名结核病患者和10名健康对照组收集了口腔抹布样本.
- 使用Illumina MiSeq测序分析了细菌16S rRNA基因V3-V4区域.
- 使用QIIME和R.进行生物信息学和统计分析.
主要成果:
- 结核病患者和对照人群之间细菌丰富度,多样性和社区组成的显著差异.
- 在结核病患者中丰富菌 (Streptococcus) 和菌 (Rothia).
- 在结核病患者中,与碳水化合物,脂肪酸和免疫信号相关的代谢途径的升级.
结论:
- 口腔细菌失生症可能在结核病发病过程中发挥作用.
- 在结核病患者中观察到口腔微生物群多样性,丰富性和功能的显著变化.
- 需要进一步的元基因组和转录基因组研究来证实这些发现.
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