在抗生素压力下高效的部分化-亚纳摩克斯 (PN/A) 过程的反应机制:细胞外聚合物,微生物群落和功能基因
Yingqiang Li1, Zhenguo Chen2, Yuexiang Huang3
1Guangdong Provincial Engineering Research Center of Intelligent Low-carbon Pollution Prevention and Digital Technology & Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, School of Environment, South China Normal University, Guangzhou 510006, People's Republic of China; SCNU (NAN'AN) Green and Low-carbon Innovation Center, Nan'an SCNU Institute of Green and Low-carbon Research, Quanzhou 362300, People's Republic of China; Huashi(Fujian) Environment Technology Co. Ltd, Quanzhou, 362001, People's Republic of China.
部分化-安纳摩克斯 (PN/A) 工艺有效地从废水中去除,即使在硫甲抗生素压力下. 这种弹性与细胞外聚合物和特定微生物群落的增加有关.
科学领域:
- 环境微生物学环境微生物学
- 废水处理技术 废水处理技术
- 生物地质化学循环是什么
背景情况:
- 废水中的抗生素可以抑制生物处理过程,如部分化-安纳摩克斯 (PN/A).
- 了解微生物对抗生素压力的反应对于保持有效的去除至关重要.
研究的目的:
- 在处理污染了抗生素的废水时,研究PN/A过程的反应机制.
- 确定关键的微生物和遗传适应,使PN/A在硫甲压力下保持过程稳定.
主要方法:
- 在硫甲压力下长期PN/A工艺培养.
- 监测去除率和关键性能指标.
- 分析细胞外聚合物质 (EPS) 和微生物群体结构 (例如,Denitratisoma,SM1A02) 的变化.
- 量化功能基因 (nirS,nirK) 和抗生素耐药性基因 (ARG) (sul1,intI1) 的丰富性.
主要成果:
- 在PN/A过程中,尽管长期暴露于硫甲,但仍然保持了高的去除率 (>1.01kg N/m3/d).
- 细胞外聚合物显著增加 (22.52至43.96毫克/克VSS),增强抗生素抑制的耐药性.
- 化物和SM1A02以及nirS和nirK基因的丰度增加,这表明化剂的作用至关重要.
- 抗生素耐药性基因 (ARG) sul1和intI1显著增加 (分别为8.78和5.12倍),有助于过程耐药性.
结论:
- 在PN/A过程中,通过增强的EPS生产和特定的微生物适应,证明了对硫甲压力的弹性.
- 脱剂和ARG的扩散是维持在抗生素压力下PN/A系统稳定的关键因素.
- 这项研究提供了在抗生素压力下PN/A过程机制的见解,指导了污染废水处理中的未来应用.
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