在高压加工下,可能在Enterococcus faecalis中转移和激活"沉默"四环素耐药基因
Patryk Wiśniewski1, Arkadiusz Zakrzewski1, Wioleta Chajęcka-Wierzchowska1
1Department of Food Microbiology, Meat Technology and Chemistry, Faculty of Food Science, University of Warmia and Mazury, Plac Cieszyński 1, 10-718, Olsztyn, Poland.
Food microbiology
|March 2, 2024
概括
高压压力可以增加四环素耐药性基因表达和转移在食物传播的Enterococcus faecalis. 即使是敏感菌株也可能在特定的压力条件下传播抗性基因,需要监测.
科学领域:
- 微生物学 微生物学
- 食品安全 食品安全
- 分子生物学分子生物学
背景情况:
- 菌 (Enterococcus faecalis) 是一种通过食物传播的细菌.
- 抗四环素耐药性在食品安全方面是一个日益关注的问题.
- 横向基因转移 (HGT) 促进了抗生素耐药性的传播.
研究的目的:
- 为了确定食物中隔离的Enterococcus faecalis.的四环素耐药性.
- 研究高压应激对四环素耐药性基因表达和HGT.的影响.
- 分析特定的四环素抗性基因 (tet基因) 的频率.
主要方法:
- 从食品样本中分离和描述Enterococcus faecalis.
- 分子分析以识别和量化四环素耐药基因 (tet(M),tet(L),tet(K),tet(W),tet(O)).
- 将细菌菌株暴露在高压应力 (400和500MPa) 中,以评估基因表达和HGT频率.
主要成果:
- 高压压力,特别是400MPa,显著增加了对四环素耐药基因的表达,特别是L).
- 基因的HGT (M,L,K,W,O)) 在对照和高压暴露菌株中得到证实.
- 与500 MPa相比,400 MPa的压力对基因表达和转移频率产生了更大的影响.
结论:
- 高压处理可以诱导表达,并促进在表型易受 Enterococcus faecalis. 在四环素耐药性基因的HGT.
- 这些发现突显了抗生素耐药性增加在食品环境中传播的潜在风险.
- 持续监测食物来源的Enterococcus faecalis分离物的四环素耐药性至关重要.
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