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甲索特雷克萨特用于药物重新定位,作为一种抗聚合剂,用于对待的α-chymotrypsinogen-A

Neha Kausar Ansari¹, Amaan Rais¹, Aabgeena Naeem²

¹Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, U.P, 202002, India.

The protein journal

|March 2, 2024

概括

甲托雷克萨特 (MTX) 是FDA批准的药物,可以逆转蛋白质聚合,这是阿尔茨海默氏症等疾病的标志. 这项研究表明MTX MTX.

科学领域:

生物化学和分子生物学
神经科学是一个神经科学.
药理学药理学是指药理学的学科.

背景情况:

蛋白质聚合与神经退行性疾病 (如阿尔茨海默氏症和帕金森病) 有关.
识别治疗剂以对抗蛋白质错折是疾病治疗的关键.

研究的目的:

研究一种现有的FDA批准药物甲索特雷克萨特 (MTX) 在逆转预制蛋白质聚合物的潜力.
探索MTX与α-chymotrypsinogen A (α-Cgn A) 聚合物相互作用和分解的机制.

主要方法:

使用离子 (Hg₂Cl₂) 诱导α-Cgn A聚合.
使用提奥夫拉T (ThT),ANS光,循环二极化 (CD),FTIR,拉曼光谱和扫描电子显微镜 (SEM) 进行聚合物的表征.
评估MTX在分解α-Cgn A聚合物的有效性和分子对接分析.

主要成果:

水银离子诱导了α-Cgn A富含β聚合物的形成,改变了它的二次结构,从α-螺旋变为β-分子间.
甲托雷克萨特有效地破坏了这些聚合物,恢复了α-Cgn A的原生结构,由减少的ThT/ANS光和恢复的α-螺旋含量证明.
分子对接揭示了MTX和聚合物中的疏水性残留物之间的特定相互作用,阐明了其作用机制.

结论:

甲托雷克萨特是一种有前途的候选药物,可用于治疗与蛋白质错折相关的疾病.
MTX在分解病态蛋白质结构方面表现出显著的有效性.
这项研究为开发针对蛋白质聚合疾病的新疗法提供了基础.

关键词:

无形聚合物是一种无形聚合物.药物重新定位是药物重新定位.甲基甲酸二醇的使用方法 α-chymotrypsinogen 一个一个一个.

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