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由IFNT诱导的IRF1通过交换激活LIFR来增强牛子宫内膜受体性

Bin Ma¹, Huiqi Cui¹, Xiao Wang¹

¹Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.

Journal of reproductive immunology

|March 3, 2024

概括

干扰素-τ (IFN-τ) 通过调节干扰素调节因子1 (IRF1) 的上调来增强牛子宫内膜受容性. 然后,IRF1增加白血病抑制因子受体 (LIFR),促进胚胎植入.

科学领域:

生殖生物学生殖生物学
内分泌学在内分泌学.
分子生物学分子生物学

背景情况:

干扰素-τ (IFN-τ) 对反动物的子宫内膜接受性至关重要,但其在牛中的确切机制尚不清楚.
干扰素调节因子1 (IRF1) 和白血病抑制因子受体 (LIFR) 分别是干扰素信号传递和胚胎植入的关键分子.

研究的目的:

调查IRF1和LIFR在IFN-τ介导的牛子宫内膜受体性中的作用.
阐明在牛子宫内膜中连接IFN-τ,IRF1和LIFR的分子通路.

主要方法:

在体内分析牛子宫内膜中的IRF1和LIFR表达在怀孕和雌性周期期间.
在体外研究中,使用用IFN-τ治疗的牛子宫内膜上皮细胞 (bEECs).
基因过度表达和干扰实验以评估IRF1和LIFR功能.
双 luciferase 记者测定以确认 IRF1 与 LIFR 促进体结合.

主要成果:

在怀孕第18天,牛子宫内膜的IRF1和LIFR表达增加.
在bEECs中IFN-τ调节了IRF1,LIFR和子宫内膜受体性标记物 (LIF,HOXA10,ITGAV,ITGB3) 的上调.
在bEEC中,IFN-τ降低了E-cadherin的表达,并减少了微的数量.
IRF1模仿了IFN-τ效应,而LIFR干扰阻断了这些效应,表明IRF1通过LIFR行动.
IRF1直接与LIFR发起人结合并进行交易.

结论:

IFN-τ在牛子宫内膜上皮细胞中诱导IRF1的表达.
通过促进体结合,IRF1通过上调LIFR表达来增强牛子宫内膜受体性.
这个IRF1-LIFR轴是IFN-τ建立子宫内膜对牛妊娠的感受性的关键机制.

关键词:

牛类牛类牛类牛类牛类牛宫内膜的受体性宫内膜的受体性国际货币基金组织-ττ 在 IRF1 中,IRF1 是 IRF1 的第一个类型.根据LIFR的规定.

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