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在Conopomorpha sinensisBradley sinensis的头部转录组中识别和描述昼夜钟基因

Lin-Fa Quan¹, Yan-Yan Chi¹, Yi-Zhi Dong¹

¹Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China.

Comparative biochemistry and physiology. Part D, Genomics & proteomics

|March 3, 2024

概括

这项研究确定了果 (Conopomorpha sinensis) 中的关键昼夜钟基因,揭示了它们在调节夜间行为 (如交配) 中的关键作用. 了解这些时钟基因为害虫控制策略提供了新的途径.

科学领域:

分子生物学分子生物学
时间生物学时间生物学
昆虫学昆虫学是一门学科.

背景情况:

Conopomorpha sinensis 是一个主要的害虫在中国的李子和龙子.
成年C. sinensis的行为,包括交配和卵置,由昼夜节律调节.
在C. sinensis中,将昼夜节律与成年人的行为联系起来的分子机制尚不清楚.

研究的目的:

在Conopomorpha sinensis中识别与昼夜钟相关的主要基因.
研究这些时钟基因的表达模式和功能作用.
为了解这一经济重要害虫的昼夜节律的分子基础提供见解.

主要方法:

使用Illumina HiSeq 6000对C. sinensis的雌雄头部进行转录组分析.
通过BLASTx进行基因注释,基于保护的分类,域架构和遗传学分析.
空间时空和昼夜节律基因表达特征的分析.

主要成果:

在C. sinensis中确定了保存的昼夜钟基因,主要表达在头部.
在发育过程中观察到显著的时空和昼夜节律积累的时钟基因.
CsinPer,CsinTim1和CsinCry1显示出动态表达模式,在第四阶段的成年人中达到顶峰,这表明他们在求爱和交配中的角色.

结论:

这项研究阐明了C. sinensis的昼夜钟基因谱.
关键的时钟基因,特别是CsinPer和CsinTim1,在调节交配行为方面有很大影响.
这些发现有助于理解果虫害害虫的节律性,并为害虫管理提供目标.

关键词:

循环时钟基因是循环时间的基因.这种动物是Conopomorpha sinensis.表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式头部的转录组头部的转录组

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