有效地将甘糖蜜转化为Tremella fuciformis多糖,通过重复批次培养提高生物活性
Caiyuan Yu1, Haipeng Zhu1, Yan Fang1
1College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
International journal of biological macromolecules
|March 3, 2024
概括
这项研究有效地从使用固定的玉米从甘糖蜜中产生了Tremella fuciformis多糖化物 (TFPS). 优化方法显著提高了TFPS产量和生产效率,产生了生物活性低分子量分数.
科学领域:
- 生物技术是生物技术.
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
背景情况:
- 特雷梅拉形多糖 (TFPS) 是一种有价值的粘多糖,在健康食品,化品和医疗材料中具有应用.
- 目前的生产方法在效率和基质利用方面面临限制.
研究的目的:
- 从农业工业残留物 (甘糖蜜) 开发生物活性TFPS重复批量生产的有效策略.
- 使用固定载体优化TFPS生产,并评估产生的多糖的特性.
主要方法:
- 使用甘糖蜜作为T. fuciformis子培养的碳来源.
- 研究了玉米作为一种固定载体,以增强子吸收并减少发酵滞后.
- 优化了TFPS生产的重复批次培养条件.
- 分析了TFPS的分子量分布和抗氧化能力.
主要成果:
- 玉米显著提高了TFPS生产效率,达到2.04g/L/d,在八个重复批次中平均产量为5.52g/L.
- 优化的发酵周期时间减少了61.61%.
- 甘糖蜜将低分子量TFPS (TFPS-2) 的比例提高到17.25%,这显示出强大的抗氧化活性.
结论:
- 这项研究为经济高效地将甘糖蜜转化为高生物活性TFPS奠定了基础.
- 开发的方法提高了TFPS生产效率,并产生了具有显著抗氧化特性的分量.
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