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对lncRNA,miRNA和mRNA表达特征的综合分析揭示了与猪丸功能相关的调节途径

Pei Wang¹, Zhipeng Liu¹, Xia Zhang²

¹College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China.

|March 3, 2024

概括

这项研究揭示了与西方品种相比,中国本土猪的丸中长非编码RNA (lncRNA) 和microRNA (miRNA) 表达的显著差异. 这些非编码RNA对于通过各种信号通路调节男性生殖功能至关重要.

科学领域:

* 生殖生物学生殖生物学
* 基因组学是一个学科.
* 分子生物学 * 分子生物学

背景情况:

*长非编码RNAs (lncRNAs) 和microRNAs (miRNAs) 是已知的哺乳动物丸功能和精子生成的调节者.
* 这些非编码RNA在猪雄性繁殖中的特定作用和调节网络在很大程度上尚未被探索.

研究的目的:

* 识别和比较中国土著Banna小猪 (BMI) 和引进的西部Duroc (DU) 和大白 (LW) 猪的丸中的lncRNA和miRNA表达特征.
*阐明差异表达的非编码RNA及其点基因在猪丸功能和雄性繁殖中的调节作用.
*构建lncRNA-mRNA,miRNA-mRNA和竞争性内源RNA (ceRNA) 网络,以了解它们的互动调节机制.

主要方法:

*RNA测序是在BMI,DU和LW猪的丸组织上进行的.
*对lncRNAs,miRNAs和mRNAs进行了差异表达分析.
*生物信息工具用于构建调节网络 (lncRNA-mRNA,miRNA-mRNA,ceRNA) 并进行功能丰富分析.

主要成果:

*中国本土和引入的西方猪品种之间的基因表达差异比引入品种之间的差异更明显.
* 在BMI和引入品种之间确定了1622个共同差异表达的基因 (共同DEG),122个共同差异表达的lncRNA (共同DEL) 和39个共同差异表达的miRNA (共同DEM).
* 在男性性功能路径 (例如,MAPK,AMPK,PI3K/Akt) 中,共同DEG和共同DEL/共同DEM的点基因被显著丰富.
* 构建了广泛的lncRNA-mRNA,miRNA-mRNA和ceRNA网络,突出了ssc-miR-1343作为一个具有众多交互因素的关键调节节点.

结论:

* lncRNAs和miRNAs在调节猪丸功能和雄性繁殖方面发挥着关键作用,土著和西方品种之间有不同的表达模式.
* 鉴定到的调节网络提供了对猪男性生育能力背后的分子机制的宝贵见解.
*这项研究为了解猪繁殖中的遗传多样性以及未来的育种策略提供了宝贵的资源.

关键词:

男人的生殖生殖器.猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪精子发生是精子生成.测试 (Testis) 是一个测试.ceRNARNA是什么意思这是一个ncRNARNA.

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