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诱导的多能干细胞衍生的细胞体对增殖和收缩的媒介做出反应

Natalie E King¹, Jo-Maree Courtney¹, Lachlan S Brown¹

¹Tasmanian School of Medicine, College of Health and Medicine, University of Tasmania, Level 4, Medical Sciences Precinct, 17 Liverpool St, Hobart, TAS, 7000, Australia.

Stem cell research & therapy

|March 3, 2024

概括

诱导的多能干细胞衍生细胞体 (iPericytes) 与人类大脑主要血管细胞体 (HBVPs) 具有功能上的相似性. 这些Ipericytes适合于神经疾病研究,提供有关皮细胞功能和功能障碍的见解.

科学领域:

血管生物学血管生物学
干细胞研究干细胞研究
神经科学是一个神经科学.

背景情况:

细胞调节大脑血流和血脑屏障的完整性.
细胞功能障碍与阿尔茨海默氏症,多发性硬化症和中风等神经系统疾病有关.
诱导多能干细胞 (iPSC) 衍生细胞 (iPericytes) 是血管研究的一个潜在工具.

研究的目的:

为了比较iPericytes的功能特征与人类大脑主要血管周细胞 (HBVPs).
评估iPericytes对于研究神经疾病病理生理学的适用性.

主要方法:

iPSCs被分化为ipericytes (中皮或神经谱系).
在ipericytes和HBVPs之间比较了形态,基因表达 (qPCR,RNA测序) 和蛋白质标记物.
功能性测试包括扩散 (PDGF-BB),收缩 (内甲素-1) 和放松 (腺素).

主要成果:

皮细胞表现出与HBVPs的形态和法典皮细胞标志物相似之处.
在iPericytes和HBVPs之间以及iPericyte血统之间观察到显著的基因表达差异.
细胞对PDGF-BB,内素-1和腺的反应与HBVPs类似,表现出增殖,收缩和放松的能力.

结论:

皮细胞表达皮细胞标记物,并表现出适当的生理反应,以mitogens和血管活性介质.
功能性Ipericytes适合未来研究神经疾病中皮质细胞的作用.

关键词:

亚丁氨酸 (Adenosine) 是一种氨基酸.契约性契约性是指契约性.输内甲林-1-1是什么人类大脑血管周细胞 (HBVPs)诱导多能干细胞 (iPSCs) 是一种干细胞.细胞 (Pericytes) 是一种细胞.血小板衍生生长因子BB (PDGF-BB)血小板衍生生长因子受体β (PDGFRβ)核武器的扩散

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